Simple and Sensitive Analysis of Blonanserin and Blonanserin C in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry and Its Application

Abstract

A highly sensitive, simple, and rapid liquid chromatography tandem mass spectrometry method to simultaneously determine blonanserin and blonanserin C in human plasma with AD-5332 as internal standard (IS) was established. A simple direct protein precipitation method was used for the sample pretreatment, and chromatographic separation was performed on a Waters XBridge C8 (4.6 × 150 mm, 3.5 μm) column. The mobile phase consists of a mixture of 10 mM ammonium formate and 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B). To quantify blonanserin, blonanserin C, and IS, multiple reaction monitoring (MRM) was performed in positive ESI mode. The calibration curve was linear in the concentration range of 0.012–5.78 ng·mL−1 for blonanserin and 0.023–11.57 ng·mL−1 for blonanserin C (r 2 > 0.9990). The intra- and interday precision of three quality control (QC) levels in plasma were less than 7.5%. Finally, the current simple, sensitive, and accurate LC-MS/MS method was successfully applied to investigate the pharmacokinetics of blonanserin and blonanserin C in healthy Chinese volunteers.

Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method

“…The liquid chromatography-electrospray ionization-tandem mass spectrometry method has many advantages. It is a simple, rapid, reliable, and effective analytical tool [33]. A new method for determination of tiliroside by LC-ESI-MS/MS was developed and applied for the first time of tiliroside.…”

Qualitative and quantitative analysis of felbinac and its major metabolites in human plasma and urine by liquid chromatography tandem mass spectrometry and its application after intravenous administration of felbinac trometamol injection

“…Matrix effect and recovery effect were examined in six replicates by comparing the peak areas of analytes between three different samples sets [16]. Stability tests involved leaving the untreated samples at ambient temperature for 6 h, placing the treated samples in auto-sampler for 20 h, three freeze-thaw cycles from −20 • C to 25 • C, and storing for 120 days at −70 • C. Stability analysis was performed using three aliquots of each QC sample at three different concentrations.…”

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“…To date procedures for determination of blonanserin [11][12][13][14][15] along with is degradant product [16][17][18][19] and in biometrics [20][21][22][23] using HPLC had been reported. In addition to this GC method for residual solvent 24,25 determination in blonanserin and blonanserin determination in human plasma 26 In addition to this Kalariya et al stated that blonanserin is thermally stable.…”