Simple and sensitive assay of zonisamide in human serum by high-performance liquid chromatography using a solid-phase extraction technique

Disposition and safety of zonisamide after intravenous and oral single dose and oral multiple dosing in normal hound dogs

“…Zonisamide was quantified in unknown samples by reverse phase HPLC (C 18 3.9 · 300 mm, 125 Å 10 lm particle size column (l-Bond-apakÔ Waters Corporation, Milford, MA, USA) with a SentryÔ Guard column, 3.9 · 20 mm (l BondapakÔ C 18, 125 Å 10 lm particle size) (SentryÔ Guard Waters Corporation, Milford, MA, USA) and ultraviolet detection at 245 nm. The mobile phase consisted of purified distilled water and methanol (70:30, v ⁄ v) with the flow rate to 1.4 mL ⁄ min (Furuno et al, 1994). Standards were prepared in canine blank serum, plasma, whole blood, and urine.…”

Simultaneous HPLC–UV analysis of rufinamide, zonisamide, lamotrigine, oxcarbazepine monohydroxy derivative and felbamate in deproteinized plasma of patients with epilepsy

“…Several HPLC methods have been published for the determination of ZNS in human plasma, coupled with UV [5][6][7][8][9][10][11], diode array [12], or mass spectrometry [13] detection, with different sample pre-treatment procedures: liquid-liquid extraction [5][6][7][8]11], solid phase extraction [12,13], or deproteinization [9,10]. Some of these methods allow for the simultaneous determination of ZNS with other AEDs, including the new generation AED levetiracetam (LEV) [8], lamotrigine (LTG) [10][11][12][13], oxcarbazepine's (OXC) main active metabolite monohydroxycarbamazepine (MHD) [10,12,13] and topiramate (TPM) [13].…”

Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring

“…HPLC assay, using a porous silica column, is commonly used for separation and quantification of many drugs, but it has been reported that 1.5∼2.0-μm particle non-porous silica columns provide superior performance over the 3.5∼10-μm particle porous silica columns in several parameters and the method on the non-porous silica column was faster, used less solvent, and provided greater analyte peak area [246][247][248]. Several HPLC methods using a conventional reversed-phase porous silica column for monitoring the serum concentration of ZSD have been reported [249,250]. However, these methods have common problems of long run time and high solvent consumption.…”