Simple and sensitive high-performance liquid chromatographic method for the investigation of dynamic changes in the redox state of rat serum albumin

Hayashi, Tomoya; Suda, Kazuhiro; Imai, Hisao; Era, Seiichi

doi:10.1016/s1570-0232(02)00068-5

Cited by 35 publications

(37 citation statements)

References 26 publications

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“…Serum concentrations of albumin were determined as described by Hayashi T et al (Hayashi et al, 2002). Briefly, 2 μl of unprocessed rat serum was injected onto Asahipak ES-502N column (100× 7.5 mm; Phenomenex Inc, Torrance, CA) maintained at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Perinatal growth restriction decreases diuretic action of furosemide in adult rats

DuBois

Pearson

Mahmood

et al. 2014

European Journal of Pharmacology

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Perinatal growth restriction programs higher risk for chronic disease during adulthood via morphological and physiological changes in organ systems. Perinatal growth restriction is highly correlated with a decreased nephron number, altered renal function and subsequent hypertension. We hypothesize that such renal maladaptations result in altered pharmacologic patterns for life. Maternal protein restriction during gestation and lactation was used to induce perinatal growth restriction in the current study. The diuretic response of furosemide (2mg/kg single i.p dose) in perinatally growth restricted rats during adulthood was investigated. Diuresis, natriuresis and renal excretion of furosemide were significantly reduced relative to controls, indicative of decreased efficacy. While a modest 12% decrease in diuresis was observed in males, females experienced 26% reduction. It is important to note that the baseline urine output and natriuresis was similar between treatment groups. The in vitro renal and hepatic metabolism of furosemide, the in vivo urinary excretion of the metabolite, and the expression of renal drug transporters was unaltered. Creatinine clearance was significantly reduced by 15% and 19% in perinatally growth restricted male and female rats, respectively. Further evidence of renal insufficiency was suggested by decreased uric acid clearance. Renal protein expression of sodium-potassium-chloride cotransporter, a pharmacodynamic target, was unaltered. In summary, perinatal growth restriction could permanently imprint pharmacokinetic processes affecting drug response.

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Section: Methodsmentioning

confidence: 99%

Perinatal growth restriction decreases diuretic action of furosemide in adult rats

DuBois

Pearson

Mahmood

et al. 2014

European Journal of Pharmacology

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“…Main outcome markers were serum albumin level and the ratio of reduced albumin (human mercaptalbumin, HMA), oxidized albumin (human non‐mercaptalbumin, HNA‐1), and irreversibly oxidized albumin (HNA‐2) within total albumin. Serum total albumin level was measured by the bromcresol green (BCG) method, and HMA, HNA‐1, and HNA‐2 were determined by high‐performance liquid chromatography (HPLC) as reported previously 11 . The HPLC profiles obtained were subjected to numerical curve fitting, and area under each peak was approximated by a Gaussian function (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…HMA, human mercaptalbumin; HNA, human non‐mercaptalbumin. Dotted lines were simulated by Gaussian function 11 …”

Section: Methodsmentioning

confidence: 99%

Oral branched‐chain amino acid supplementation improves the oxidized/reduced albumin ratio in patients with liver cirrhosis

Fukushima¹,

Miwa²,

Shiraki³

et al. 2007

Hepatology Research

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“…HPLC was performed as described by Hayashi et al (20). Sera samples obtained from each rat were immediately frozen and stored at j80-C until used for HPLC analysis.…”

Section: Chromatographic Analysis Of Serum Albumin In Ratsmentioning

confidence: 99%

“…The level of RNA/RMA ratio was estimated by the redox states of 34-Cys of rat serum albumin because Cys is highly accessible to reactive oxygen species. Therefore, the level of RNA/RMA ratio represents an appropriate marker of oxidative stress in CRF (20).…”

Section: Chromatographic Analysis Of Serum Albumin In Ratsmentioning

confidence: 99%

An Oral Adsorbent, AST-120 Protects Against the Progression of Oxidative Stress by Reducing the Accumulation of Indoxyl Sulfate in the Systemic Circulation in Renal Failure

Shimoishi

Anraku²,

Kitamura³

et al. 2007

Pharm Res

104

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Purpose. The effect of AST-120, an oral adsorbent, on oxidative stress in the systemic circulation in chronic renal failure (CRF) was examined and the potential role of indoxyl sulfate (IS), an uremic toxin adsorbed by AST-120, in inducing the formation of reactive oxygen species (ROS) in the vascular system was studied, in vitro and in vivo. Materials and methods. The level of oxidized albumin, a marker for oxidative stress in the systemic circulation was determined by HPLC, as previously reported. The mRNA levels of TGF-b 1 and Oat1 were measured by quantitative RT-PCR. The IS induced ROS generation in cultured human umbilical vein endothelial cells (HUVECs) was estimated using a fluorescence microplate reader.Results. An increase in the ratio of oxidized to unoxidized albumin was determined using 5/6 nephrectomized rats (CRF rats) compared to a control group. The ratio was significantly reduced in the group that received AST-120 of 4 weeks, suggesting that AST-120 inhibits oxidative stress in CRF. An anti-oxidative effect of AST-120 was also observed in CRF rats with a similar renal function. The ratio of oxidized albumin was correlated with serum IS levels in vivo. The same relationship was also observed in CRF rats with the continued administration of IS. In addition, IS dramatically increased the generation of ROS in both a dose-and time-dependent manner in HUVEC, suggesting that accumulated IS may play an important role in enhancing intravascular oxidative stress. Conclusion. We propose that AST-120 reduces IS concentrations in the blood that induces ROS production in endothelial cells, thereby inhibiting the subsequent occurrence of oxidative stress in the systemic circulation in renal failure.

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Simple and sensitive high-performance liquid chromatographic method for the investigation of dynamic changes in the redox state of rat serum albumin

Cited by 35 publications

References 26 publications

Perinatal growth restriction decreases diuretic action of furosemide in adult rats

Perinatal growth restriction decreases diuretic action of furosemide in adult rats

Oral branched‐chain amino acid supplementation improves the oxidized/reduced albumin ratio in patients with liver cirrhosis

An Oral Adsorbent, AST-120 Protects Against the Progression of Oxidative Stress by Reducing the Accumulation of Indoxyl Sulfate in the Systemic Circulation in Renal Failure

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