Simple Cannulation Procedure for Serial Blood Sampling Through Cutaneous Ulnar Vein in Chickens

Bayer, Darmel M; Mohan, Kiran; Jayakumar, K.; Manafi, M.; Pavithra, B. H.

doi:10.1080/10888705.2012.624925

Cited by 6 publications

(7 citation statements)

References 6 publications

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“…According to the results obtained, the studied chickens were divided into three groups in accordance with the ANS department tone: sympathicotonics (St), normotonics (Nt) and vagotonics (Vt), four animals in each group. At the age of 60 days, blood was drawn from the saphenous vein of the chickens' shoulder (Bayer, 2012). Blood was sampled from 10 am to 1 pm, after a short fasting (2:00).…”

Section: Methodsmentioning

confidence: 99%

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Interactions of productivity, content of separate amino acids, general protein in blood serum of chickens and tone of the autonomic nervous system

Studenok

Shnurenko

Karpovskyi

et al. 2021

Regul. Mech. Biosyst.

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The regulation of metabolic processes and maintenance of a stable internal environment of the body is the role of the autonomic nervous system (ANS). It influences all systems and organs, thus along with the neuro-humoral system providing control over the metabolism of proteins, fats and carbohydrates. Amino acids, as structural units of any protein molecule, are essential for the metabolism of proteins, enzymes and hormones. Certain amino acids are involved in neutralizing free radicals and are a donor of metal groups, can act as an energy source in case of a deficiency of carbohydrates and fats, and be structural units of connective tissue. Exchange of these compounds and their regulation by ANS have not been studied sufficiently, therefore, it is necessary to carry out research in this direction. The studies were aimed at finding the presence of the regulatory effect of ANS on the content of individual amino acids in the blood serum of 60-day-old Cobb-500 chickens. Determination of the ANS tone in Cobb-500 chickens at the age of 30–35 days was determined by the method of variation pulsometry. Electrocardiographic examination was performed in a quiet room without the use of sedatives. At the age of 60 days, blood was obtained from the saphenous vein of the chickens’ shoulder. Blood was sampled from 10 am to 1 pm, after a short fasting (2:00). It was found that sympathicotonic chickens had a higher content of individual amino acids compared to normotonics and vagotonics. In vagotonic chickens, the content of glycine and serine in the blood serum significantly exceeded the rate of chickens with a balanced tone of ANS. Correlative relationships between amino acid content were highly significant in poultry with balanced ANS tone. Vagotonic chickens had the highest correlations between ANS tone, heart rate and amino acid content (trend). Correlative relationships were present between ANS and body weight of chickens of different groups, serum protein. The correlation between total serum protein and body weight of chickens was different depending on the tone of the ANS. Fisher’s test revealed a significant effect of balanced ANS tone on the content of valine, serine and glycine and the weight of chickens. The effect of ANS tone on weight in vagotonic birds was high.

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Section: Methodsmentioning

confidence: 99%

“…Blood was sampled from 10 am to 1 pm, after a short fasting (2:00). The chickens were put in the supine position, the vein puncture site was treated aseptically and anesthetized with lidocaine gel (Bayer, 2012). The amino acid content in blood serum was determined using "Kapel 105М" (Russia) by capillary electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Interactions of productivity, content of separate amino acids, general protein in blood serum of chickens and tone of the autonomic nervous system

Studenok

Shnurenko

Karpovskyi

et al. 2021

Regul. Mech. Biosyst.

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“…Experimental animals, depending on the tone of the ANS were divided into 3 groups: sympathicotonics, in which the indicators of the mode of the duration of the cardiac cycle (Mo) were 0.14-0.16 s, the amplitude of the mode (Amo) -˃ 45%; normotonics (Mo -0.16-0.17 s, Amo -40-45%); vagotonics (Mo -0.18-0.21 s, Amo -˂ 40%) (Studenok et al, 2020). Venous blood for biochemical studies was taken from the subcutaneous vein of the shoulder after a 2-hour fasting diet (Bayer et al, 2012). The venipuncture site was cleaned of dirt, disinfected with 70% aqueous solution of ethyl alcohol, skin anesthesia was performed with gel (Kategel, Austria) based on lidocaine (Bayer et al, 2012).…”

Section: Materials and Methods Of Researchmentioning

confidence: 99%

Dynamics of Serum Protein Content and Productivity of Chickens With Different Tonus of the Autonomic Nervous System

Studenok

Shnurenko

Трокоз

et al. 2021

Ukrainian journal of veterinary sciences

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The main role in maintaining the functioning of the body, its growth, and development belongs to protein. It is involved in the formation of the muscular skeleton and is s part of enzymes, neurotransmitters, hormones. The effect of the autonomic nervous system on total protein metabolism has not been sufficiently studied. It is known that the autonomic nervous system is a structure that is responsible for the homeostasis and stability of the whole organism. It participates in the regulation of the heart, endocrine and external secretion glands, gastrointestinal tract, excretory organs, and more. In our studies, it was found that in chickens of Cobb 500 strain with different tones of the autonomic nervous system during the growing period from the 35th to the 60th day, different contents of total protein, albumin, and globulins were observed and different body weights were recorded. Vagotonic chickens showed the lowest protein metabolism at the age of 35 and 45 days (P ˂ 0.05–0.001) compared with sympathicotonics and normotonics, which tended to increase between 35 and 60 days of rearing compared with other groups of birds, where the studied protein fractions on the contrary decreased. Correlations between total protein, albumin, and bird body weight had a high linear relationship in all groups of chickens (P ˂ 0.05–0.001) and a negative relationship between the 45th and 60th days of rearing in sympathicotonics and normotonics. In birds with a predominance of parasympathetic tone of the autonomic nervous system, this correlation maintained its direction with high reliability (P ˂ 0.05) between body weight and total protein on the 60th day of rearing.

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“…Birds were maintained under environmental temperature of 24 • C and 20% RH ( Figure 1A). Birds were cannulated in the cutaneous ulnar vein on the right wing, using the technique of Bayer et al (2012). Briefly, feathers were removed, and the area surrounding the vein was cleaned with 70% ethanol.…”

Section: Animal Procedures and Environmentmentioning

confidence: 99%

Monitoring Phytate Hydrolysis Using Serial Blood Sampling and Feather Myo-Inositol Levels in Broilers

et al. 2020

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Phytate forms insoluble precipitates with various cations that are recalcitrant to digestion in poultry. Dietary supplementation with exogenous phytase has been shown to improve phytate solubility and digestibility and, in turn, improve animal growth performance. Although the kinetics of phytate hydrolysis by exogenous phytase are well described in vitro, the progression of the reaction in vivo is still not well defined. The aim of the present study was, therefore, to monitor the kinetic variation of myo-inositol (myo-Ins) levels in both circulation and feather following exogenous phytase supplementation. In experiment 1, 4 week-old male broilers were individually housed with ad libitum access to water and a standard commercial diet. Birds were maintained under environmental temperature of 24 • C and 30% RH. Birds were cannulated in the cutaneous ulnar vein on the right wing and remained untouched for 3 days. On the day of the experiment, birds were randomly divided into three body weight-matched groups and fed either the control diet, the control diet-supplemented with myo-Ins or Ronozyme HiPhos (0.06%, DSM Nutritional Products, Switzerland) for 10 h. In the experiment 2, birds were fed only HiPhos for 30 h. Growing feathers and blood were collected at baseline and then every 2 h for 10 h (experiment 1) and 30 h (experiment 2) post-prandially. Plasma and feather myo-Ins levels were determined by UHPLC-MS/MS. The relative expression of inositol polyphosphate-1-phosphatase (INPP1), inositol hexakisphosphate kinase 1-3 (IP6K1-3), inositol-3-phosphate synthase (ISYNA), and multiple inositol-polyphosphate phosphatase 1 (MNPP1) genes in blood and feathers was determined by real-time qPCR using 2 − Ct method. Plasma and feather myo-Ins levels were significantly increased by HiPhos at 6 h to 8 h post-prandial. The mRNA abundances of INPP1, IP6K1, and ISYNA in the circulation were significantly down regulated at all periods compared to the baseline levels. IP6K2, IP6K3, and MINPP1 gene expression, however, was up regulated at 8 h post-prandial and then returned to the baseline levels. In feathers, the expression of INPP1 was induced at 8 h post-prandial and remained higher compared to the baseline. The expression of IP6K2, IP6K3, and MINPP1 was

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Simple Cannulation Procedure for Serial Blood Sampling Through Cutaneous Ulnar Vein in Chickens

Cited by 6 publications

References 6 publications

Interactions of productivity, content of separate amino acids, general protein in blood serum of chickens and tone of the autonomic nervous system

Interactions of productivity, content of separate amino acids, general protein in blood serum of chickens and tone of the autonomic nervous system

Dynamics of Serum Protein Content and Productivity of Chickens With Different Tonus of the Autonomic Nervous System

Monitoring Phytate Hydrolysis Using Serial Blood Sampling and Feather Myo-Inositol Levels in Broilers

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