Simple chemiluminescence determination of ketotifen using tris(1,10 phenanthroline)ruthenium(II)‐ Ce(IV) system

Abstract

A new method using chemiluminescence (CL) detection has been developed for the simple determination of ketotifen fumarate (KF). The method is based on the catalytic effect of KF in the CL reaction of tris(1,10 phenanthroline)ruthenium(II), Ru(phen)3 (2+), with Ce(IV) in sulfuric acid medium. The CL response was detected using a lab-made chemiluminometer. Effects of chemical variables were investigated and under optimum conditions, the CL intensity was proportional to the concentration of the drug over the range 0.34-34.00 µg mL(-1) KF. The limit of detection (S/N=3) was 0.09 µg mL(-1). Effects of common ingredients were investigated and the method was applied successfully for determining KF in pharmaceutical formulations and human plasma. The percent of relative standard deviation (n=11) at level of 3.4 µg mL(-1) of KF was 4.6% and the minimum sampling rate was 70 samples per hour. The possible CL mechanism is proposed based on the kinetic characteristic of the CL reaction, CL spectrum, UV-Vis and phosphorescence spectra.

Determination of pholcodine in syrups and human plasma using the chemiluminescence system of tris(1,10 phenanthroline)ruthenium(II) and acidic Ce(IV)

“…In this way, the analytical signal could be free from blank interferences from substances such as ascorbic acid and cysteine in human plasma. We used this strategy successfully for the determination of carminic acid, ketotifen, aspirin and, recently, hydroxyzine and mepivacaine in real samples. As can be seen in Figure S4 in Supporting Information, the CL kinetic profile of plasma did not have a detectable response at 10 sec after injection of Ce(IV) solution.…”

Synthesis of neodymium-iron nanoperovskite for sensing applications of an antiallergic drug

“…Hang ng mercury drop electrode [36] 0.2 Not reported 8 × 10 -7 to 8 × 10 -5 mol L -1 Not reported Chem lum nescence Tr s(1,10 phenanthrol ne) ruthen um(II)-Ce(IV) system [37] 0.5 Not reported 7 × 10 -7 to 6 × 10 -5 mol L -1 Not reported Spectrophotometr c Ion-pa r w th bromothymol blue [38] 2.92 nmol L -1 310.6 µA/mmol L -1 0.8 µmol L -1 to 360 µmol L -1…”

Chemiluminescence Determination of Local Anaesthetic Mepivacaine in Human Plasma and Pharmaceuticals

“…In this way, analytical signal could be free of blank interferences of some substances such as ascorbic acid and cysteine in human plasma. We used this strategy successfully for determination of carminic acid, 33 ketotifen, 34 aspirin, 35 and hydroxyzine in real samples. As can be seen in Figure 5, CL kinetic profile of plasma does not have a detectable response at 10 s after injection of Ce(IV) solution.…”