Simple determination of bromide ion in urine by head space gas chromatography.

Sugiyama, Naokazu; Saito, Kin-ichi; Nomoto, Akemi; Hanabusa, Takashi; Satô, Hisashi; Kawai, Mika

doi:10.2116/bunsekikagaku.34.6_335

Cited by 6 publications

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“…Numerous methods have been reported for the determination of bromide in blood and/or urine using ion chromatography [8], gas chromatography (GC) [1,2,[9][10][11][12][13], radio activation analysis [3], inductively coupled plasma mass spectrometry [14], X-ray spectrometry [15], cyclic voltammetry [16] and photometry [17].…”

Section: Introductionmentioning

confidence: 99%

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Determination of bromide in whole blood and urine from humans using gas chromatography–mass spectrometry

Kage¹,

Kudo

Ikeda

et al. 2005

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

“…Majority of these methods, however, identify bromide only by the retention time [1,2,[8][9][10][11][12][13] or the absorbance [17], thus they lack specificity. Gas chromatography-mass spectrometry (GC-MS) is a popular and specific method for forensic toxicological examination.…”

Section: Introductionmentioning

confidence: 99%

Determination of bromide in whole blood and urine from humans using gas chromatography–mass spectrometry

Kage¹,

Kudo

Ikeda

et al. 2005

Journal of Chromatography B

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“… 5 However, most of these methods require a large amount of samples, and as the methods are complicated and time‐consuming, they are inconvenient as emergency diagnostic methods. Although a simple method for determining Br − concentration in urine using headspace gas chromatography was developed, 6 which was suitable for the analysis of volatile substances and has been used for biological monitoring at industrial sites, it showed poor reproducibility and a low recovery rate when blood samples were tested directly. Yamano et al reported a simple method for determining Br − concentration in the blood, with some modifications.…”

Section: Introductionmentioning

confidence: 99%

Quantification of bromide ion in biological samples using headspace gas chromatography–mass spectrometry

Yamauchi

Nagashima

Okubo

et al. 2021

Journal of Occupational Health

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Objectives In this study, we aimed to establish a method for quantifying bromide ions (Br − ) in blood and urine using gas chromatograph‐mass spectrometer (GC‐MS) equipped with a headspace sampler, for biological monitoring of workers exposed to methyl bromide. Methods Samples were mixed with dimethyl sulfate, and Br − ions were detected using GC‐MS with a headspace sampler. The validity of the proposed method was evaluated based on most of the US FDA guidance. The values obtained were compared with reference values by analysis using Seronorm TM Trace Elements Whole Blood L‐1 RUO. Results The calibration curve showed good linearity in the Br − concentration range of 0.1−20.0 mg/L, and the coefficient of determination R 2 value was >.999. Intraday and interday accuracy values were 99.3%−103.1% and 97.4%−101.8%, respectively. The measured and reference values of Seronorm were concordant. Herein, eight urine and serum samples of workers were analyzed; the samples' Br − concentrations were known. The correlation coefficients of urine and serum samples were 0.97 and 0.96, respectively, and results were consistent. Conclusions This study established a simple and rapid method for the determination of Br − concentration in biological samples using GC‐MS with a headspace sampler. Moreover, it can be used for biological monitoring of occupational exposure to methyl bromide and for the determination of Br − concentration in a wide range of biological samples.

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Experimental exposure of rat skin to methyl bromide: a toxicokinetic and histopathological study

Yamamoto¹,

Hori

Tanaka

et al. 2000

Archives of Toxicology

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Methyl bromide was experimentally exposed to a 12 cm2 area of the back skin of Wistar rats for 30 s, and for 1, 3, and 5 min, and time courses of both changes in plasma bromide concentration and of histopathological changes were examined. To measure bromide ion, a head space gas chromatography was used. The concentration of plasma bromide ion showed a sharp increase immediately after the exposure in all exposed groups, reaching a peak level after 1 h, then decreased rapidly. The ion level gradually decreased after 72 h to 1 week, and returned to a normal level after 4 to 8 weeks. Calculating from a regressive curve, the biological half lives of plasma bromide ion were 5.0 days to 6.5 days. Histopathologically, the impairments to the epidermal cells, fibroblasts and blood vessels were observed in the early phase. These cellular changes could be due to the direct cytotoxicity of the compound. In the next phase, newly infiltrating cells showed degeneration and necrosis. Subsequently, an impairment of the collagen bundles was observed. Our experiments suggested an immediate permeation and rapid metabolization of methyl bromide in the skin and a multistep formation of the skin damage induced by the compound. These processes of methyl bromide-induced skin damage are quite different from chemical skin injuries caused by the representative causative agents such as alkaline and acid.

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Simple determination of bromide ion in urine by head space gas chromatography.

Cited by 6 publications

References 0 publications

Determination of bromide in whole blood and urine from humans using gas chromatography–mass spectrometry

Determination of bromide in whole blood and urine from humans using gas chromatography–mass spectrometry

Quantification of bromide ion in biological samples using headspace gas chromatography–mass spectrometry

Experimental exposure of rat skin to methyl bromide: a toxicokinetic and histopathological study

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