Simple Embryo Isolation Techniques from Ophrys (Orchidaceae) Seeds

Abstract

Investigations on embryos and seeds are very difficult, owing to the fact that orchid seeds are very small, dusty, and transparent. Therefore, this study looked at how to prepare the isolation technique for orchid seeds and how to isolate orchid embryos from seeds. The seeds of Barlia robertiana (Orchidaceae) were used. Consequently, for orchid seeds, the most suitable isolation method was established and its practical importance is discussed.