Simple high-performance liquid chromatographic method for the determination of acyclovir in pharmaceuticals

Basavaiah, K.; Prameela, H. C.; Chandrashekar, U.

doi:10.1016/s0014-827x(03)00157-5

Cited by 18 publications

(20 citation statements)

References 21 publications

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“…The author concluded that those methods are simple and rapid. They also concluded that their spectrophotometric method did not need any heating or extracting [13].…”

Section: 4mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

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“…The author concluded that those methods are simple and rapid. They also concluded that their spectrophotometric method did not need any heating or extracting [13].…”

Section: 4mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

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“…The determination of amlodipine level in biological samples has been reported with high performance liquid chromatography with ultraviolet (HPLC-UV) [6][7][8][9] , and mass spectrometric detector [10][11][12][13][14] . Although several methods have been reported for the determination of amlodipine in plasma, these methods have low sensitivity and narrow range of linearity [10][11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Liquid Chromatography - Tandem Mass Spectrometry Method for Determination of Amlodipine in Human Plasma and Its Application

Pongnarin¹,

Thanakosai²,

Kingkhangphloo³

et al. 2017

Pharm Sci Asia

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A sensitive liquid chromatography tandem mass spectrometry method was developed to quantify amlodipine in human plasma. Alkalinized plasma spiked with desipramine, an internal standard, was extracted by liquid-liquid extraction and evaporated an organic part to dryness. The residue was reconstituted and injected into an Acquity Ultra Performance LC TM , (Waters, Co., Ltd. USA) with C 18 column. The isocratic elution of mobile phase was performed by 90% of acetonitrile and 10% of 10 mM ammonium acetate pH 4 at flow rate of 0.20 mL/min with 5 minutes of total run time. Mass spectrometric analysis was performed using a Quattro Premier XE mass spectrometer, (Micromass Technologies, UK) coupled with an electrospray ionization (ESI) source in the positive ion mode. The MRM transitions of m/z 409.17>238.19 and 409.17>294.09 were selected for amlodipine and 267.09>208.06 for desipramine. The retention times were 1.63 and 1.69 minutes for amlodipine and desipramine, respectively. The linearity of the method revealed a correlation coefficient of >0.998 within the concentration range of 0.05 -20 ng/mL. This work has been fully validated according to the Guidance for Industry: Bioanalytical Method Validation (USFDA CDER, 2001, BP) with high degree of accuracy and precision. This method was applied to quantify amlodipine concentrations in human plasma samples in a bioequivalence study.

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“…In many studies, LNZ was used as the main analyte or as internal standard (IS), in that HPLC [1,2] by UV detection and UV spectroscopic methods were used, which were not suitable for clinical trials because of their low sensitivity [14][15][16][17][18][19][20][21][22][23][24][25][26]. Thus, the objective of the project was to develop and validate suitable method for estimation of unknown concentration of drug in plasma.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a LC-MS/MS Method to Determine Lansoprazole in Human Plasma

Kachave¹,

Kale²,

Wagh³

2015

TOACJ

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A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for the determination of lansoprazole (LNZ) in human plasma. Pantaprazole was used as an internal standard (IS). Analytes and the internal standard were extracted from human plasma by solid-phase extraction technique using Oasis HLB, Oasis Max, Varian Bond Elute Plexa, Orochem cartridges. The reconstituted samples were chromatographed on a thermo hypurity Advance, 50 X 4.6mm, 5 µ by using acetonitrile and 2 mM ammonium acetate solution(80:20 v/v) as the mobile phase at a flow rate of 1.0 mL/min. Detection was carried out LC-MS/MS (API 3000) in negative ion mode. The calibration curves obtained were linear (R 2 -0.999) over the concentration range of 4.50-2800.00 ng/ml for lansaprazole. The results of the intra-and inter-day precision studies were well within the acceptable limits. The overall average recoveries of analyte and IS were found to be 92.10-99.11%. The analyte were found to be stable of stability study. Developed and validated analytical method was found to be simple, rapid, specific, sensitive, precise and cost effective than reported methods. The method has been successfully applied to the investigation of a preclinical pharmacokinetic study with desired precision and accuracy along with high throughput.

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Simple high-performance liquid chromatographic method for the determination of acyclovir in pharmaceuticals

Cited by 18 publications

References 21 publications

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Development and Validation of Liquid Chromatography - Tandem Mass Spectrometry Method for Determination of Amlodipine in Human Plasma and Its Application

Development and Validation of a LC-MS/MS Method to Determine Lansoprazole in Human Plasma

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