Simple manipulation of enzyme-linked immunosorbent assay (ELISA) using an automated microfluidic interface

Abstract

Among lateral flow immunoassay (LFIA) platforms, enzyme-based LFIAs use signal amplification to improve sensitivity. However, most enzyme-based LFIAs require multiple timed steps, complicating their use in point-of-care testing (POCT). Here,...

3D-printed capillaric ELISA-on-a-chip with aliquoting

“…However, LFAs offer only qualitative yes-no results, their sensitivity is typically lower compared to that of laboratory ELISA and are not suitable for archival as the readout must be completed within a few minutes of the test, because otherwise, the result can be compromised. [10][11][12] Enzymatic amplification has been implemented in the LFAs, 13,14 for instance by using a microfluidic interface, 14 to improve sensitivity. Yet, they cannot implement various fluidic handling tasks of common ELISA such as timed incubation of reagents and multiple rinsing steps between each incubation interval.…”

Sequential Flow Controllable Microfluidic Device for G-Quadruplex DNAzyme-Based Electrochemical Detection of SARS-CoV-2 Using a Pyrrolidinyl Peptide Nucleic Acid

“…Recently, the capillary-driven microfluidic device was introduced as a new class of microfluidic platforms by Henry’s group and has been increasingly used for POC applications. − The devices were easily fabricated by laminating patterned hydrophilic polyester and double-sided adhesive films to generate narrow gaps, whereby the fluid transport occurs via the capillary force without requiring an external pump . Such devices possess several desirable characteristics of POC devices including simple fabrication, controllable solution flow, nonrequirement of external pumps, and sequential reagent delivery . To add further levels of control to the capillary-driven microfluidic devices, Jang et al recently introduced a burst valve system as a new flow control method .…”

“…36 Such devices possess several desirable characteristics of POC devices including simple fabrication, controllable solution flow, nonrequirement of external pumps, and sequential reagent delivery. 40 To add further levels of control to the capillary-driven microfluidic devices, Jang et al recently introduced a burst valve system as a new flow control method. 36 The burst valve was designed to control the liquid flow by the use of a different gap to stop the fluid flow.…”

Trimetallic Au@Pd@Pt nanozyme-enhanced lateral flow immunoassay for the detection of SARS-CoV-2 nucleocapsid protein

“…Various detection methods, including the enzyme-linked immunosorbent assay (ELISA), 10 electrochemical sensors, 11,12 and lateral ow immunoassay (LFIA), 13 have been explored for the detection of the SARS-CoV-2 N-protein. Among these methods, LFIA is a kind of widely accepted method for its advantages of being rapid, portable, affordable, and suitable for large-scale detection.…”