Simple method for the determination of rhein in biological fluids by high-performance liquid chromatography

Springolo, V.; Coppi, G

doi:10.1016/s0378-4347(00)83904-3

Cited by 14 publications

(7 citation statements)

References 6 publications

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“…A previous study showed drug stability and HPLC profile of diacerhein and its metabolite rhein in the serum and urine of treated rats [50,51]. The original data of qualitative HPLC results presented here allowed us to identify the presence of diacerhein in pancreas and serum of treated animals, confirming drug absorption by the organ tissue.…”

Section: Discussionsupporting

confidence: 65%

Diacerhein downregulate proinflammatory cytokines expression and decrease the autoimmune diabetes frequency in nonobese diabetic (NOD) mice

Malaguti

Vilella

Vieira

et al. 2008

International Immunopharmacology

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Section: Discussionsupporting

confidence: 65%

Diacerhein downregulate proinflammatory cytokines expression and decrease the autoimmune diabetes frequency in nonobese diabetic (NOD) mice

Malaguti

Vilella

Vieira

et al. 2008

International Immunopharmacology

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“…Concentrations of unchanged rhein were measured in plasma and urine using the high-performance liquid chromatographic method of Springolo and Coppi (1988) with minor modifications. Briefly, I ml of the biological sample was deproteinised with an equal volume of acetonitrile.…”

Section: Discussionmentioning

confidence: 99%

Influence of renal function on the pharmacokinetics of diacerein after a single oral dose

Louchahi

Tod

Molinier

et al. 1993

Fundamemntal Clinical Pharma

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The pharmacokinetics of diacerein (a new anti-inflammatory analgesic antipyretic drug) following a single oral dose of 50 mg was studied in 12 healthy volunteers and two groups of eight patients with mild or severe renal insufficiency. Statistical analysis using a Kruskal-Wallis rank sum test showed a significant difference between the three groups for the following parameters. In severely uraemic patients, median AUC0-infinity was multiplied by a factor of ca 2: 40.5 mg h/l versus 21.3 in healthy subjects, P = 0.04; and t1/2 was prolonged by the same factor: 9.6 h versus 4.3 in the control group, P = 0.003. Apparent drug availability and renal clearance assessed through urinary data decreased with renal failure, respectively: 14.5% and 0.045 l/h versus 35.4% (P = 0.01) and 0.13 l/h (P = 0.008) in healthy subjects. Amounts of glucuro and sulfo conjugates in urine were lower in severely uraemic patients. Intermediate values were observed for mildly uraemic patients. Other parameters: lag-time, Cmax, tmax, Vss/F, urinary glucuro- to sulphoconjugate ratios did not change significantly. Apparent total clearance of rhein was poorly correlated with creatinine clearance and this was related to a decrease of non-renal clearance of rhein in renal insufficiency. It was concluded that, from a pharmacokinetic point of view, a reduction (50%) in the maintenance dosage of diacerein should be considered in severe renal failure.

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“…The pharmacokinetics of rhein have been reported by many researchers (Springolo and Coppi, 1988). In humans, rhein has also been detected in the plasma after the administration of Agiolax and Sennatin (Krumbiegel and Schulz, 1993).…”

Section: Introductionmentioning

confidence: 88%

Determination and pharmacokinetic comparison of rhein in rats after oral dosed with Da‐Cheng‐Qi decoction and Xiao‐Cheng‐Qi decoction

Tang

Huang

et al. 2007

Biomedical Chromatography

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Da-Cheng-Qi decoction (DCQD) is composed of dahuang, houpu, zhishi and mangxiao, while Xiao-Cheng-Qi decoction (XCQD) just contains dahuang, houpu, and zhishi. A validated high-performance liquid chromatographic (HPLC) method was developed for the determination and pharmacokinetic comparison of rhein in rats. It was performed on a reversed-phase C(18) column (150 x 4.6 mm) with a mobile phase of methanol and 0.2% acetic acid (89:11, v/v) and ultraviolet detection at 254 nm. The assay was linear over the range 30-3000 ng/mL (r(2) = 0.9991), with mean percentage recovery of 101.4%. The RSD of intra- and inter-day precision were less than 3.0%, with accuracy in the range 96.8-102.5% for each of the concentrations tested. Following oral administration, rhein plasma levels revealed curves characterized by peaks at 20.5 and 30.1 min corresponding to dosages of 15 mg/kg (XCQD) and 20 mg/kg (DCQD) at mean concentrations of 1184.67 and 1814.27 ng/mL, respectively. The half-lives of rhein from XCQD and DCQD were 45.3 and 139.6 min, respectively. AUC(0-12h) of rhein after oral dosage with XCQD and DCQD were 3435.68 and 5382.25 microg/L h, respectively. The pharmacokinetic parameters may be used to guide the clinical prescription compatibility of traditional Chinese prescriptions related to dahuang and mangxiao.

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Simple method for the determination of rhein in biological fluids by high-performance liquid chromatography

Cited by 14 publications

References 6 publications

Diacerhein downregulate proinflammatory cytokines expression and decrease the autoimmune diabetes frequency in nonobese diabetic (NOD) mice

Diacerhein downregulate proinflammatory cytokines expression and decrease the autoimmune diabetes frequency in nonobese diabetic (NOD) mice

Influence of renal function on the pharmacokinetics of diacerein after a single oral dose

Determination and pharmacokinetic comparison of rhein in rats after oral dosed with Da‐Cheng‐Qi decoction and Xiao‐Cheng‐Qi decoction

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