Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography

Abstract

A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367 cm h(-1), resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3-4. The isocratic elution with 0.15 M NaCl led to approx. >90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield.

Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives

“…Various strategies for the purification of bacteriocins from complex cultivation broths have exploited their cationic and hydrophobic characteristics (Cheigh et al, 2004). Usual methods for bacteriocins extraction are based on their affinity to organic solvents, their variation in solubility in concentrated salt solutions and at a given pH value.…”

“…Bacteria from this group are involved in the production of the lantibiotic nisin, lactococcins, lactostrepcins, diplococcins and others (Mackay et al, 1997;Moreno et al, 2000). Cheigh et al (2004) have developed a simple onestep purification method, using expanded bed ionexchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164.…”

Large-scale purification of a bacteriocin produced by Leuconostoc mesenteroides subsp. cremoris using diatomite calcium silicate

“…Laboratory purification procedures include an ammonium sulfate precipitation step, followed by various combinations of cation-exchange and hydrophobic interaction chromatography, with a final reverse-phase chromatography (Parente and Ricciardi, 1999). Although the current purification methods work well for low volumes, they can be difficult to scale up when working with the large volumes needed for large-scale industrial production (Uteng et al, 2002;Cheigh et al, 2004). In this study, the bacteriocin produced by Lc.…”

Optimization of a Culture Medium Composition as Aproach to Isolation of Bacteriocins

“…These purifi cation methods require expensive equipment at each step, and may result in a signifi cant loss of desired product [6]. An increase in the effectiveness of liquid chromatography methods, as well as a reduction in the number of purifi cation steps may be due to the cultivation of producers on media with a minimum content of proteins and other hydrophobic components that impede the release of bacteriocins [7].…”