Simple, speedy, sensitive, and specific serodiagnosis of pertussis by using a particle agglutination test

Abstract

We developed a particle agglutination test (KPA) with poly(␥-methyl L-glutamate) as the solid particle for measurement of pertussis toxin (PT) antibody. In this study, KPA was assessed as a means of serodiagnosing pertussis, and the results were compared with those of indirect enzyme-linked immunosorbent assay (indirect ELISA) and the microagglutination test. First, four serum samples were collected from each of 21 healthy children: before and 4 weeks after receiving three primary doses of acellular pertussis vaccines and before and 4 weeks after receiving a booster dose. In all 21 vaccinees, a significant rise in PT antibody titers was observed by KPA after each vaccination, and among all 84 serum samples collected, an excellent correlation was demonstrated between the values obtained by indirect ELISA and those obtained by KPA (r ‫؍‬ 0.92). Second, paired serum samples were collected at intervals of approximately 2 weeks from 51 patients with cultureconfirmed pertussis. A significant increase in titer (fourfold or more) was observed in 39 (76%) patients by KPA, 34 (67%) patients by indirect ELISA, and 23 (45%) patients by the microagglutination test. In acute-and convalescent-phase sera collected from 20 nonpertussis patients, there were no changes in titers by KPA. The KPA procedure was as simple as that of the microagglutination test, and the reaction time was only 2 h (or overnight). In this study, KPA was demonstrated to be a simple, speedy, sensitive, and specific serodiagnostic method for pertussis.

Speedy, Sensitive, and Specific Diagnosis of Pertussis by Using Serum and the Sensitized-Particle Test

“…In their recent communication, Aoyama et al (1) reported that they were successful in obtaining a serological diagnosis of pertussis by using Bordetella pertussis toxin (PT)-sensitized porous, spherical, poly(␥-methyl-L-glutamate) particles. The particle agglutination test (KPA) was standardized for detection and quantification of immunoglobulin G (IgG) against PT by comparison of serological results with those obtained by indirect enzyme-linked immunosorbent assay and the microagglutination test.…”