Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel

Wu, Yingji; Jin, Xin; Afshar, Elham Ashrafzadeh; Taher, Mohammad Ali; Xia, Changlei; Joo, Sang‐Woo; Mashifana, Tebogo; Vasseghian, Yasser

doi:10.1016/j.chemosphere.2022.135392

Cited by 10 publications

(2 citation statements)

References 36 publications

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“…In addition to the optical signal generation, they are responsible for the immobilization of affinity molecules and the maintenance of the 3D structure of the biosensor. In general, fluorescent gels are obtained by incorporating fluorescent labels, such as organic dyes [32], proteins/peptides [33], aromatic hydrocarbons [34], metal nanoparticles [35], or NCs [36], into the gel structure. The main requirements for fluorescent labels are the capability for multiplexed detection, high detection sensitivity and fluorescent signal intensity, photo-stability, and a reliable and convenient procedure for immobilizing fluorescent labels in the hydrogel.…”

Section: Approaches To Obtaining Fluorescent Gelsmentioning

confidence: 99%

Biosensors Based on Inorganic Composite Fluorescent Hydrogels

et al. 2023

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Fluorescent hydrogels are promising candidate materials for portable biosensors to be used in point-of-care diagnosis because (1) they have a greater capacity for binding organic molecules than immunochromatographic test systems, determined by the immobilization of affinity labels within the three-dimensional hydrogel structure; (2) fluorescent detection is more sensitive than the colorimetric detection of gold nanoparticles or stained latex microparticles; (3) the properties of the gel matrix can be finely tuned for better compatibility and detection of different analytes; and (4) hydrogel biosensors can be made to be reusable and suitable for studying dynamic processes in real time. Water-soluble fluorescent nanocrystals are widely used for in vitro and in vivo biological imaging due to their unique optical properties, and hydrogels based on these allow the preservation of these properties in bulk composite macrostructures. Here we review the techniques for obtaining analyte-sensitive fluorescent hydrogels based on nanocrystals, the main methods used for detecting the fluorescent signal changes, and the approaches to the formation of inorganic fluorescent hydrogels via sol–gel phase transition using surface ligands of the nanocrystals.

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Section: Approaches To Obtaining Fluorescent Gelsmentioning

confidence: 99%

Biosensors Based on Inorganic Composite Fluorescent Hydrogels

et al. 2023

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“…Один из основных компонентов ФГГ -ФМ, которые и придают им флуоресцентные свойства. В качестве таких меток могут быть использованы органические ФК [21], ФБ и пептиды [22], ароматические углеводороды [23], металлические наночастицы [24], а также квантовые точки (КвТ) и другие НК [25]. Основные требования к ФМ заключаются в возможности мультиплексной детекции, высокой интенсивности флуоресцентного сигнала (ФС), фотостабильности, а также возможности надежной и удобной иммобилизации ФМ внутри ГГ.…”

Section: свойства флуоресцентных гидрогелей и способы их формированияunclassified

Principles and approaches in the development of fluorescent hydrogels for cancer diagnosis

Sokolov,

Samokhvalov,

Baranova

et al. 2023

Rossijskij bioterapevtičeskij žurnal

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Introduction. Early sensitive and highly specific diagnosis is crucial for successful cancer therapy. The use of fluorescent hydrogels (FHG) makes it possible to develop versatile biosensors due to the increased binding capacity of biological capture and reporter molecules, sensitive fluorescence detection, and the flexibility of combining their structural and functional elements.Aim. Analyzing the principles of designing biosensors based on FHG for the detection of cancer markers and the methodological approaches to their development, as well as summarizing and systematizing the data on the principles of detection and target signal generation used in these sensors.Results. FHG represent 3D sensing platforms, i. e., structures that combine the reporter fluorescence function with biological capture molecules, allowing the unique optical properties of fluorescent nanocrystals at the macro level to be preserved. The porous structure of hydrogels increases the active surface area of biosensors for 3D immobilization of fluorescent labels and biological capture molecules, while preserving the structure of these molecules, which ensures specific binding of the detected molecules of the sample. This ensures a higher sensitivity compared with the traditional methods of immunoenzymatic and immunochromatographic analyses. Not only the traditionally used antibodies, but also enzymes and glycoproteins, aptamers and oligonucleotides, as well as polymers obtained by molecular imprinting, can serve as biological capture molecules, which extends the range of specifically detectable analytes.Conclusion. The review presents examples of biosensors based on FHG intended for the detection of cancer markers and describes approaches to the preparation of FHG and immobilization of biological capture molecules, as well as principles of generation of the detected optical signal. The main advantages of fluorescent hydrogel biosensors over the classical tests used for quick diagnosis of cancer are shown.

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Application of gold nanoclusters in fluorescence sensing and biological detection

Tan,

Ma,

et al. 2024

Anal Bioanal Chem

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Simple turn-off fluorescence sensor for determination of raloxifene using gold nanoparticles stabilized by chitosan hydrogel

Cited by 10 publications

References 36 publications

Biosensors Based on Inorganic Composite Fluorescent Hydrogels

Biosensors Based on Inorganic Composite Fluorescent Hydrogels

Principles and approaches in the development of fluorescent hydrogels for cancer diagnosis

Application of gold nanoclusters in fluorescence sensing and biological detection

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