Simple Validated Method for Determination of Deoxynivalenol and Zearalenone in Some Cereals Using High Performance Liquid Chromatography

Sebaei, Ahmed Salem; Gomaa, Ahmed M.; El-Di, F.A. Nour

doi:10.3923/ajft.2012.668.678

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…The solution was centrifuged for five minutes. 10 ml of supernatant was loaded on solid phase extraction column conditioned with 20 ml ethyl acetate for elution and the eluent was evaporated and dissolved to dryness with mobile phase before HPLC analysis [29].…”

Section: Mycotoxin Extraction and Analysismentioning

confidence: 99%

Fungal and Mycotoxin Contamination of Stored Maize in Kogi, Northcentral Nigeria: An Implication for Public Health

Akoma

Ezeh

Chukwudozie

et al. 2019

EJNFS

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The maize value chain in the Kogi State and most parts of the country from where maize is purchased into the State lacks mechanisms that ensure grain quality and safety. Against the above-backdrop, this study was designed to evaluate toxigenic fungi and associated mycotoxins in maize produced within different agro-zones of Kogi State. Harvested and stored maize seeds under different storage conditions were collected from three different zones (Zone B Bassa, Zone C Lokoja, and Zone D Idah) and cultured. Different fungal species were isolated by culturing using the spread plate technique on potato dextrose agar (PDA) and identified microscopically. Mycotoxin production by isolated fungi was subsequently evaluated for Deoxynivalenol (DON) contamination using the High-Performance Liquid Chromatography technique (HPLC). The outcome of the study was statistically analysed using simple frequencies and percentages. Aspergillus spp. and Penicillium spp. were the fungi found to be associated with the stored seeds in Kogi, while Fusarium spp. Mucor spp. and Rhizopus spp. were the field fungi identified. Of the thirteen samples collected, the most common genera were Aspergillus (isolated from 41.67% of the evaluated samples), Fusarium (27%) and in a lesser extent Rhizopus spp. (8.33%). The result also shows DON was detected in 92.3% of the stored maize samples, making it one of the widespread mycotoxin contaminants of maize grain. Implications of this study for human and animal health and economic development were discussed and appropriate recommendations made especially for adoption of proper storage technology among small-scale farmers for improved maize quality and safety.

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Section: Mycotoxin Extraction and Analysismentioning

confidence: 99%

Fungal and Mycotoxin Contamination of Stored Maize in Kogi, Northcentral Nigeria: An Implication for Public Health

Akoma

Ezeh

Chukwudozie

et al. 2019

EJNFS

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“…Thus, a rapid, robust, and reliable UPLC-FLD method was developed in this study for the simultaneous determination of AFB1 and AFM1 residues in broiler tissues. The method is novel, sensitive, and accurate in the sense that simultaneous determination of AFB1 and AFM1 residues in liver, kidney, and muscle tissues has been performed for the first time using immunoaffinity column in comparison with other reported laborious, less sensitive, complex, and expensive methods (Potesil et al, 2005 ; Petrlova et al, 2006 ; World Health Organisation, 2006 ; Han et al, 2010 ; Sebaei et al, 2012 ; Decleer et al, 2016 ). The following tests were performed to optimize the method according to the standard guidelines (Epshtein, 2004 ) and choose the most appropriate UPLC-FLD conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Samples properly prepared gives results free from interfering peaks. Previously, it has been stated that polar solvents are the efficient solvents used for extracting mycotoxins and water provides higher extraction efficiencies in mixtures, by enhancing penetration of the solvent (Hinojo et al, 2006 ; Sebaei et al, 2012 ). In this study, different extraction solvents were tested to facilitate the extraction of AFB1 and AFM1 residues from broiler tissues, however, dichloromethane was selected as the best extraction solvent due to its highest recoveries for both AFB1 and AFM1 residues (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

Cui

Ishfaq

Li³

et al. 2017

Front. Pharmacol.

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Aflatoxin B1 (AFB1) and its metabolite aflatoxin M1 (AFM1) are well-known carcinogens for humans and animals health. In this study, an ultra-high performance liquid chromatography linked with fluorescence detection (UPLC-FLD) method was optimized and validated. In addition, we investigated for the first time, the influence of curcumin on residue depletion of AFB1 and AFM1 in liver, kidney, and muscle tissues of broiler chickens and estimated a necessary clearance time required for AFB1 and AFM1 residues. The results showed that the average recoveries of AFB1 varied in liver, kidney, and muscles between 82.32–85.56, 85.34–88.45, and 84.88–89.73% respectively, while the average recoveries of AFM1 in liver, kidney, and muscles varied between 92.17–95.03, 94.12–97.21, and 95.32–98.51%, respectively. The detection limit of aflatoxin B1 was 0.008 ng/ml, while for aflatoxin M1 was 0.003 ng/ml. The limit of quantification (LOQ) for AFB1 and AFM1 was 0.02 and 0.01 ng/ml, respectively. Clearance time for AFB1 and AFM1 residues were analyzed in two experimental groups of broilers. One group fed with dietary AFB1 (5.0 mg/kg feed) and other with curcumin+AFB1 diet (curcumin; 300 mg/kg feed, AFB1; 5.0 mg/kg feed). AFB1 and AFM1 residues clearance time was calculated based on LOQ using withdrawal time calculation software (WT1.4). Clearance time analyzed for AFB1 ranged from 11 to 19 days and for AFM1 ranged from 10 to 12 days at 95% confidence level. Interestingly, curcumin supplementation in the diet reduced the clearance time of AFM1 in liver and kidney but not in muscle tissues. Conclusively, the developed method can be appropriately used for the quality control testing of commercial broiler-meat processing companies, food manufacturers, and quality control laboratories.

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“…Relatively polar solvents are the most efficient solvents that have been used for extracting mycotoxins and water offers higher extraction efficiencies in mixtures, by increasing penetration of the organic solvent [25, 26]. Since AFM1 is very slightly soluble in water [2, 27], a suitable amount of water (80 mL) was added for extraction from egg tissues and it is the lowest amount facilitating the flow over the manifold system without blocking the immunoaffinity column taking into account the heavy density and viscosity of egg samples.…”

Section: Resultsmentioning

confidence: 99%

Reliable HPLC Determination of Aflatoxin M1 in Eggs

Khalil

Gomaa²,

Sebaei³

2013

Journal of Analytical Methods in Chemistry

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Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recovery of aflatoxin M1 at the three different levels 0.05, 0.1, and 0.5 μg/kg varied between 87% and 98%. The method is linear from the limit of quantification 0.05 μg/kg up to 3 μg/kg levels. This method is intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg.

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Simple Validated Method for Determination of Deoxynivalenol and Zearalenone in Some Cereals Using High Performance Liquid Chromatography

Cited by 13 publications

References 20 publications

Fungal and Mycotoxin Contamination of Stored Maize in Kogi, Northcentral Nigeria: An Implication for Public Health

Fungal and Mycotoxin Contamination of Stored Maize in Kogi, Northcentral Nigeria: An Implication for Public Health

Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

Reliable HPLC Determination of Aflatoxin M1 in Eggs

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