Simpler technique for serological detection of H–Y antigen on mouse lymphocytes

Human H-Y: a Male-Specific Histocompatibility Antigen Derived from the SMCY Protein

“…Both protems share significant sequence homology to retinoblastoma binding protein 2, which has been suggested to be a transcnp-1590 bCIENCE non factor (26). If SMCY functions as such, its presumed intracellular location would be inconsistent with detection by male-specif-IC antibodies that have been shown to recognize cell surface structures (27) Although the function of SMCY, as well as the homologous SMCX, remains unclear, this and other H-Y specific peptides are candidates for immunornodulatory approaches in bone marrow transplantation, genetic probes to be used for prenatal diagnosis in sex-hnked congenital abnormalities, and mvestigating minimal residual disease and chimensm. G. Cacalano et al desenbe neutrophil and Β cell expansion in mice lacking the murine interleukin-8 receptor homolog (mIL-8Rh) (I).…”

H ‐ Y antigen in human intersexuality

“…H-Y antiserum was raised in C57BL/6 female mice by five weekly intraperitoneal injections of spleen cells (4.5 x 10';) from C57BL/6 male mice. Earlier it was known that, for some unknown reason, anti-H-Y antibody was not directly cytotoxic for male mouse lymphocytes, but very recently Tokuda et al (1977) have demonstrated the presence of H-Y antigen from lymphocytes of male mice. However, to date no relevent information (i.e.…”

Primary sex determination

“…Representative vertebrate species showing distribution of sex-specific H-Y antigen identified by serum from male-sensitized female mice kinds of serologic assays for H-Y antigen. These involve 1) tactics in which target cells are labeled with visual markers such as tobacco mosaic virus or sheep red blood cells (6)(7)(8)(9), or fluorescent markers (10,l l), and 2) hemagglutination (12).…”