Simplified Determination of Serum Iron and Total Iron-Binding Capacity

Abstract

2,4,6-Tripyridyl-s-triazine (TPTZ) is a color reagent for ferrous ion in the pH range 2 to 10, when ascorbic acid is used as the reducing agent. In this range TPTZ and ferrous ion combine in the ratio of two to one. Tris buffer is commonly used to maintain an alkaline pH, but it reacts with other reducing agents, rendering them ineffective. These observations suggested a method for the joint determination of serum iron and total iron-binding capacity (TIBC), all done in a single tube and with one color reagent. The method is compared with those of Peters et al. The standard deviation of the difference between the serum iron methods is less than 6 µg/100 ml, between the TIBC methods, less than 19 µg/100 ml.

Super‐Absorbent Polymer Valves and Colorimetric Chemistries for Time‐Sequenced Discrete Sampling and Chloride Analysis of Sweat via Skin‐Mounted Soft Microfluidics

“…The approach incorporated here exploits a well‐known colorimetric assay commonly used for the determination of chloride ion concentration in applications involving wastewater and biofluid media. This assay relies on competitive chelation between mercury‐(II), Hg 2+ , and ferrous‐(II), Fe 2+ , using 2,4,6‐tris(2‐pyridyl)‐s‐triazine (TPTZ) as a chelating agent . In a solution containing TPTZ (chelator), Hg 2+ and Fe 2+ , stoichiometric complexation between Hg 2+ and TPTZ is favored, resulting in the formation of colorless Hg [TPTZ] 2 (Figure S5, Supporting Information).…”