1998
DOI: 10.3748/wjg.v4.i1.74
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Simplified isolation and spheroidal aggregate culture of rat hepatocytes

Abstract: AIM To explore a simplified method for isolation of hepatocytes and establish a method of primary hepatocyte culture with more aggregates and longer persisence. METHODS Wistar rat hepatocytes were isolated by a single extracorporeal two-step perfusion method, and the cells were seeded on poly-HEMA coated flasks and cultured with hormonally defined medium and gentle shaking at regular intervals. RESULTS The total yield of isolating hepatocytes amounted to 10 8 cells for each rat liver with the viability of more… Show more

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Cited by 9 publications
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