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AIM To explore a simplified method for isolation of hepatocytes and establish a method of primary hepatocyte culture with more aggregates and longer persisence. METHODS Wistar rat hepatocytes were isolated by a single extracorporeal two-step perfusion method, and the cells were seeded on poly-HEMA coated flasks and cultured with hormonally defined medium and gentle shaking at regular intervals. RESULTS The total yield of isolating hepatocytes amounted to 10 8 cells for each rat liver with the viability of more than 90% in all isolations. Under the nonadherent environments, the cells were found to attach to each other and form multicellular aggregates rapidly, and the aggregates became spheroidal shape after two days in culture. The morphologic characteristics and albumin synthetic function of the multicellular spheroidal aggregates can be maintained for one month. CONCLUSION The simple and reliable isolation as well as large scale and longer time culture of hepatocytes can be used for experiments in liver cell transplantation and bioartificial liver support system.

With the construction and improvement of 5G infrastructure, more devices choose to access the Internet to achieve some functions. People are paying more attention to information security in the use of network devices. This makes lightweight block ciphers become a hotspot. A lightweight block cipher with superior performance can ensure the security of information while reducing the consumption of device resources. Traditional optimization tools, such as brute force or random search, are often used to solve the design of Symmetric-Key primitives. The metaheuristic algorithm was first used to solve the design of Symmetric-Key primitives of SKINNY. The genetic algorithm and the simulated annealing algorithm are used to increase the number of active S-boxes in SKINNY, thus improving the security of SKINNY. Based on this, to improve search efficiency and optimize search results, we design a novel metaheuristic algorithm, named particle swarm-like normal optimization algorithm (PSNO) to design the Symmetric-Key primitives of SKINNY. With our algorithm, one or better algorithm components can be obtained more quickly. The results in the experiments show that our search results are better than those of the genetic algorithm and the simulated annealing algorithm. The search efficiency is significantly improved. The algorithm we proposed can be generalized to the design of Symmetric-Key primitives of other lightweight block ciphers with clear evaluation indicators, where the corresponding indicators can be used as the objective functions.

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