Simplified Method for Producing Pyocins from Pseudomonas aeruginosa

Jones, Lois Faye; Pinto, Barbara; Thomas, Evan T.; Farmer, J. J.

doi:10.1128/aem.26.1.120-121.1973

Cited by 10 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pyocin production. Pyocins were produced in pyocin production medium 81 by the simplified method recently described (22). Briefly, the procedure was as follows.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Pyocin Typing of Pseudomonas aeruginosa: a Simplified Method

Jones¹,

Zakanycz²,

Thomas³

et al. 1974

Applied Microbiology

Self Cite

View full text Add to dashboard Cite

A simplified method has been devised for typing Pseudomonas aeruginosa by pyocin production. Pyocins are produced as strains grow overnight in Trypticase soy broth (without glucose) plus 1% potassium nitrate. Because P. aeruginosa can use nitrate instead of oxygen as a terminal electron acceptor, mechanical shaking is not necessary, nor is induction by mitomycin C. Pyocins can now be produced in screw-cap tubes in a water bath or incubator. A total of 250 strains were tested as possible pyocin indicators, which included 60 strains already used in pyocin-typing systems. The final set contained 18 indicators which were chosen because (i) they had clear positive or clear negative reactions, thus eliminating reactions difficult to read, (ii) they had few zones due to bacteriophage lysis, and (iii) they were most sensitive in differentiating clinical isolates of P. aeruginosa. The final typing method was tested in several studies and the results were clear; thus definitive epidemiological conclusions could be made. Because it is simple to perform and easily automated, the new method should have application in many hospitals; however, it should be used only in carefully planned epidemiological studies. The method and its application are described in detail, and some pitfalls are discussed. Nosocomial infections caused by Pseudomonas aeruginosa continue to be a problem in many hospitals (3, 5, 8, 12, 16, 23). Within any institution there can be hundreds of different strains because it is often found in the human gut, and because it can survive and multiply in moist environments of the hospital. In any epidemiological study, a sensitive technique is required to compare these numerous strains. A number of methods have been used to differentiate P. aeruginosa in epidemiological studies. These epidemiological markers include

show abstract

“…Pyocin production. Pyocins were produced in pyocin production medium 81 by the simplified method recently described (22). Briefly, the procedure was as follows.…”

Section: Methodsmentioning

confidence: 99%

“…We recently described a simplified method of producing pyocins (22) which should be applicable in most laboratories. Although the new method worked with all of the sets of pyocin indicators currently in use, some gave much clearer reactions than others.…”

mentioning

confidence: 99%

Pyocin Typing of Pseudomonas aeruginosa: a Simplified Method

Jones¹,

Zakanycz²,

Thomas³

et al. 1974

Applied Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…On a mesuri I'augmentation des cellules dans des populations de P. aeruginosa, incubies en aerobiose 1 32 "C en bouillon peptone (BP) et en BP additionni de 200 000 uap/ml, en titrant les unitis formatrices de colonies par ml (ufc/ml) dans des aliquotes prises chaque 30 minutes. 6. Antibiogramme de souches de P. aerrrginosa.…”

Section: Introductionunclassified

Relations entre résistance aux antibiotiques et résistance à la pyocine P₃ en mutants de *Pseudomonas aeruginosa**

Wilckens

1983

Zentralblatt für Veterinärmedizin Reihe B

View full text Add to dashboard Cite

Résumé On a isolé 9 clons mutants résistants á une pyocine produit par la souche P. aeruginosa P3. Ces mutants modifient leur pyocinotype par rapport aux souches sauvages d'origine et son comportement face à 4 antibiotiques d'usage thérapeutique tels que: penicilline, lyncomicine, oxacycline et tetracycline est aussi altéré. L'étude plus approfondi de ces mutants peut contribuer à comprendre les mécanismes d'action léthifère des pyocines. Summary Relations between resistance to antibiotics and resistance to pyocin P3 in mutants of Pseudomonas aeruginosa Nine mutant clones were isolated which were resistant against a pyocin product of P. aeruginosa strain P3. These mutants changed their pyocin type in relation to the original wild strain and also changed their behaviour towards four antibiotics used for therapeutic purposes — penicillin, lincomycon, oxacycline and tetracycline. A study in depth of these mutants offers some contribution towards an understanding of the lethiferous action of pyocins. Zusammenfassung Beziehungen zwischen dem Antibiotikawiderstand und dem Widerstand gegenüber Pyozin3 bei Pseudomonas aeruginosa‐Mutanten Es wurden 9 mutante Klone isoliert, die gegenüber dem P. aeruginosa‐Stamm P3 resistent sind. Diese Mutanten ändern ihren Pyozintyp mit Bezug auf die ursprünglichen wilden Stämme ab, und ebenfalls verändert sich ihr Verhalten gegenüber 4 Antibiotika mit therapeutischer Anwendung: Penicill***in, Lincomycin, Oxacyclin und Tetracyclin. Eine gründlichere Untersuchung dieser Mutanten könnte dazu beitragen, die Mechanismen der lethipheren Wirkung der Pyozinen zu verstehen. Resumen Relaciones entre la resistencìa frente a los antibióticos y la resistencia a la piocina P3 en mutantes de Pseudomonas aeruginosa Se aislaron 9 clones mutantes resistentes a una piocina producida por la estirpe P. aeruginosa P3. Estas mutantes modifican su piocinotipo en relación con las cepas salvajes de origen y también se halla alterado su comportamiento cara a los 4 antibióticos de uso terapéutico: penicilina, lincomicina, oxaciclina y tetraciclina. El estudio más a fondo de estas mutantes podría contribuir a comprender los mecanismos de acción letífera de las piocinas.

show abstract