Simulated biological fluid exposure changes nanoceria’s surface properties but not its biological response

Yokel, Robert A.; Hancock, Matthew L.; Cherian, Benjamin; Brooks, Alexandra J.; Ensor, Marsha L.; Vekaria, Hemendra J.; Sullivan, Patrick G.; Grulke, Eric A.

doi:10.1016/j.ejpb.2019.09.023

Cited by 17 publications

(17 citation statements)

References 94 publications

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“…Although a protein corona formation around this material is highly likely, its effect may be relatively small, possibly because AEROSIL ® OX50 had the smallest specific surface and the smallest SEAR’s number. Of note, the addition of protein to nanoparticles does not change or attenuate their biologic activity in general, as shown, e.g., for CeO 2 [ 43 ], TiO 2 or Fe 2 O 3 [ 24 ]. Therefore, the specific surface reactivity of a given material needs to be taken into account if the effect of protein coating has to be predicted.…”

Section: Discussionmentioning

confidence: 99%

“…The question of which type of protein coating in vitro adequately mimics the situation of particles in the lung is still unresolved. While it is beyond dispute that nanoparticles in body fluids such as blood or extracellular fluid carry a protein corona [ 13 , 43 , 45 ], protein corona formation in the lung parenchyma is more complex. At least in theory, a respirable (SAS) particle will first contact and adsorb biomolecules of the lung surfactant (phospholipids, various surfactant proteins) before it enters into the lung lining fluid with its multitude of different proteins [ 46 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

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Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner

Wiemann¹,

Vennemann²,

Venzago

et al. 2021

Nanomaterials

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Various cell types are compromised by synthetic amorphous silica (SAS) if they are exposed to SAS under protein-free conditions in vitro. Addition of serum protein can mitigate most SAS effects, but it is not clear whether this is solely caused by protein corona formation and/or altered particle uptake. Because sensitive and reliable mass spectrometric measurements of SiO2 NP are cumbersome, quantitative uptake studies of SAS at the cellular level are largely missing. In this study, we combined the comparison of SAS effects on alveolar macrophages in the presence and absence of foetal calf serum with mass spectrometric measurement of 28Si in alkaline cell lysates. Effects on the release of lactate dehydrogenase, glucuronidase, TNFα and H2O2 of precipitated (SIPERNAT® 50, SIPERNAT® 160) and fumed SAS (AEROSIL® OX50, AEROSIL® 380 F) were lowered close to control level by foetal calf serum (FCS) added to the medium. Using a quantitative high resolution ICP-MS measurement combined with electron microscopy, we found that FCS reduced the uptake of particle mass by 9.9% (SIPERNAT® 50) up to 83.8% (AEROSIL® OX50). Additionally, larger particle agglomerates were less frequent in cells in the presence of FCS. Plotting values for lactate dehydrogenase (LDH), glucuronidase (GLU) or tumour necrosis factor alpha (TNFα) against the mean cellular dose showed the reduction of bioactivity with a particle sedimentation bias. As a whole, the mitigating effects of FCS on precipitated and fumed SAS on alveolar macrophages are caused by a reduction of bioactivity and by a lowered internalization, and both effects occur in a particle specific manner. The method to quantify nanosized SiO2 in cells is a valuable tool for future in vitro studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner

Wiemann¹,

Vennemann²,

Venzago

et al. 2021

Nanomaterials

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“…There are, however, other kinds of in vivo formed NPs that can be directly associated with the invader NPs. These can form when dissolution reactions release ions from disintegrating NPs, ,, and released ions can become the nutrient supply to nucleate and form secondary NPs; , examples of secondary NPs are nanoneedles of cerium phosphate that form after the in vivo dissolution of cerium oxide. The secondary NPs may have different size, shape, and surface areas compared with the starting materials.…”

Section: Advanced Imaging and Analysis Of Nanoparticles In Tissue Sec...mentioning

confidence: 99%

Tissue Specific Fate of Nanomaterials by Advanced Analytical Imaging Techniques - A Review

Graham

Dozier

Oberdörster

et al. 2020

Chem. Res. Toxicol.

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“…Inferences from PubMed: Fourier-transform infrared spectroscopy (FTIR) is a method extensively employed [115][116][117] in biomedical, life sciences, and neuroscience. It aims to derive an infrared spectrum of absorption or emission of a solid, liquid, and gas.…”

Section: Tools For Frequency Domain Analysis Fourier Analysismentioning

confidence: 99%

Utilization of Time Series Tools in Life-sciences and Neuroscience

2020

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Time series tools are part and parcel of modern day research. Their usage in the biomedical field; specifically, in neuroscience, has not been previously quantified. A quantification of trends can tell about lacunae in the current uses and point towards future uses. We evaluated the principles and applications of few classical time series tools, such as Principal Component Analysis, Neural Networks, common Auto-regression Models, Markov Models, Hidden Markov Models, Fourier Analysis, Spectral Analysis, in addition to diverse work, generically lumped under time series category. We quantified the usage from two perspectives, one, information technology professionals’, other, researchers utilizing these tools for biomedical and neuroscience research. For understanding trends from the information technology perspective, we evaluated two of the largest open source question and answer databases of Stack Overflow and Cross Validated. We quantified the trends in their application in the biomedical domain, and specifically neuroscience, by searching literature and application usage on PubMed. While the use of all the time series tools continues to gain popularity in general biomedical and life science research, and also neuroscience, and so have been the total number of questions asked on Stack overflow and Cross Validated, the total views to questions on these are on a decrease in recent years, indicating well established texts, algorithms, and libraries, resulting in engineers not looking for what used to be common questions a few years back. The use of these tools in neuroscience clearly leaves room for improvement.

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Simulated biological fluid exposure changes nanoceria’s surface properties but not its biological response

Cited by 17 publications

References 94 publications

Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner

Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner

Tissue Specific Fate of Nanomaterials by Advanced Analytical Imaging Techniques - A Review

Utilization of Time Series Tools in Life-sciences and Neuroscience

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