Simulated gastrointestinal digest from preserved egg white exerts anti-inflammatory effects on Caco-2 cells and a mouse model of DSS-induced colitis

Zhao, Yan; Yao, Yao; Xu, Mingsheng; Wang, Shuzheng; Wang, Xiong; Tu, Yonggang

doi:10.1016/j.jff.2017.06.028

Cited by 42 publications

(23 citation statements)

References 38 publications

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“…Our results showed that the expressions of IL-10 and EGF in the high-, middle-, and low-dose KFXL groups were higher than those in the DSS-induced group, while the expression of IL-1β and IL-17 were lower. The data suggest that KFXL can regulate the balance of pro-inflammatory and anti-inflammatory cytokines and promote tissue repair, which is consistent with the previous reports [ 17 , 38 ].…”

Section: Discussionsupporting

confidence: 93%

Kangfuxin Liquid Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Ulcerative Colitis in Mice by Modulating Immune Response and Suppressing Inflammation

Tang

et al. 2021

Med Sci Monit Basic Res

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Background The aim of this study was to determine the effect of kangfuxin liquid (KFXL) on inflammatory response, and its underlying mechanism in treating acute ulcerative colitis (UC) in mice induced by dextran sulfate sodium (DSS). Material/Methods Mice were provided drinking water containing DSS (3%) for 7 days to induce acute enteritis. The mice were divided into 6 groups: a control group, a DSS-induced (vehicle) group, a sulfasalazine (SASP) group, and low-, medium-, and high-dose kangfuxin liquid groups. Disease activity index (DAI), colon mucosa damage index (CMDI), histopathological score (HS), and organ index were monitored daily. The levels of interleukin-1β (IL-1β), interleukin-10 (IL-10) in serum and interleukin-17 (IL-17) and epidermal growth factor (EGF) in colon tissue were assessed by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was used to assess the changes of T lymphocyte subsets in spleens of mice to evaluate the therapeutic effect of drugs on acute UC in mice. Results Different doses of kangfuxin liquid reduced the DAI, CMDI, and HS scores ( P <0.01 or P <0.05) of acute UC mice, reduced the level of IL-1β and IL-17 in serum, increased the expression of IL-10 in serum and EGF in colon tissue, increased the number of CD3 + T cells, and decreased the level of CD4 + T cells and the ratio of CD4 + /CD8 + . Conclusions Kangfuxin liquid has a therapeutic effect on DSS-induced acute UC in mice, and its mechanism of action may be associated with regulating immune function and reducing intestinal inflammatory response.

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Section: Discussionsupporting

confidence: 93%

Kangfuxin Liquid Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Ulcerative Colitis in Mice by Modulating Immune Response and Suppressing Inflammation

Tang

et al. 2021

Med Sci Monit Basic Res

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“…Although significant differences exist in the level of TNF‐ α of serum among three egg white groups, there were no significant differences in the TNF‐ α expression in the cecal tissue of three egg‐white groups (data not shown), which may indicate that the intake of different egg whites may affect the antigen load of healthy rats through the intestinal flora but it could not affect the levels of inflammatory cytokines in the gut. However, an earlier study had found that simulated gastrointestinal digestion from preserved egg white was better in inhibiting the expression of pro‐inflammatory cytokines and exerts anti‐inflammatory effects on DSS‐induced colitis in rats . This may be due to the fact that the rats used were normal rats rather than rat models of inflammation.…”

Section: Discussionmentioning

confidence: 98%

“…However, an earlier study had found that simulated gastrointestinal digestion from preserved egg white was better in inhibiting the expression of pro-inflammatory cytokines and exerts antiinflammatory effects on DSS-induced colitis in rats. 19 This may be due to the fact that the rats used were normal rats rather than rat models of inflammation. It is also worth noting that the gut flora were less rich and diverse in the PEW group, which may be related to the copper ions in the preserved egg white and different peptides in its digestion products.…”

Section: Discussionmentioning

confidence: 99%

“…The intake of egg proteins also has the capability to regulate the body's physiology. Simulated gastrointestinal digests from duck egg white and preserved egg white exert anti-inflammatory effects, 19 but the role played by gut microbes in this process is still unknown. It has been reported that pepsin hen egg white hydrolysate can ameliorate obesity-related oxidative stress and inflammation in Zucker fatty rats, 20 but the impact of hen egg white intake on gut microbes is still unclear.…”

Section: Introductionmentioning

confidence: 99%

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A comparative study of the modulation of the gut microbiota in rats by dietary intervention with different sources of egg‐white proteins

Qiu

Meng

et al. 2020

J Sci Food Agric

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BACKGROUND Gut microflora dysbiosis has been related closely to a variety of diseases including obesity and inflammatory bowel disease. Proteins and peptides in egg white have been found to alleviate inflammation but the role played by the intestinal flora is still unclear. Using casein as a dietary protein control, we investigated the effects of diets composed of hen egg white, duck egg white, and preserved egg white on gut microbiota in the rat cecum. RESULTS The gut microbiota in rats were altered after egg‐white consumption. The results showed that rats fed with egg white had a similar overall structure of cecal bacterial communities, which was different from those fed with casein. The relative abundance of Akkermansia in the group of rats fed with hen egg white was highest among all groups. Rats fed with duck egg white had significantly higher relative abundance of Proteobacteria and Peptostreptococcaceae, and lower relative abundance of Lachnospiraceae. In addition, the levels of lipopolysaccharide (LPS) and lipopolysaccharide‐binding protein (LBP) in rats fed with duck egg white and preserved egg white were lower than the levels in rats fed with hen egg white. CONCLUSION Our results indicated the possible positive effect on homeostasis of the intestinal flora brought about by the intake of duck egg white. This study provides an insight into the potential positive impact of preserved egg white on health by changing gut microbiota and affecting the antigen load. © 2020 Society of Chemical Industry

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“…The immune response is controlled by a complex interplay of various cytokines ( 11 ). Some proinflammatory cytokines and mediators (e.g., TNF-a, IL-6 and IL-1β) have been used as markers of anti-inflammatory activity in a dextran sodium sulfate colitis mouse model ( 51 , 52 ), and IL-10 has anti-inflammatory properties and functions in immune regulation ( 53 ). Previous studies have found that dietary MCE supplementation decreased serum IL-6 and IL-1β concentrations and inhibited the progress of inflammatory disease ( 54 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Combined Supplementation of Macleaya cordata Extract and Benzoic Acid on the Growth Performance, Immune Responses, Antioxidant Capacity, Intestinal Morphology, and Microbial Composition in Weaned Piglets

et al. 2021

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Because the use of antibiotics is forbidden, piglets experience a considerable weanling stress, resulting in increased incidence of diarrhea and death. Macleaya cordata extract or benzoic acid have anti-inflammatory, antioxidant, and antimicrobial activities that makes them potential antibiotic alternatives. The objective of this study was to evaluate the potential effects of feed supplemented with Macleaya cordata extract and benzoic acid on growth performance, immunity, antioxidant capacity, intestinal morphology, and microflora in weaned piglets. Twenty-four weaned piglets [Duroc × (Large White × Landrace)] 28 days of age and weighing 8.41 ± 0.13 kg were randomly divided in equal numbers (n = 8) into three groups fed a basal diet (CON), CON + 20 mg/kg flavomycin + 50 mg/kg quinocetone (AGP), or CON + 50 mg/kg Macleaya cordata extract + 1,000 mg/kg benzoic acid (MB). Compared with the CON diet, dietary MB or AGP increased the final weight and average daily gain, and reduced feed efficiency and the diarrhea rate (P < 0.05). Compared with the CON diet, MB supplementation increased serum superoxide dismutase (SOD activity) and decreased malondialdehyde (MDA) content (P < 0.05). Serum interleukin (IL)-10 IgA and IgM were higher (P < 0.05) in MB-fed piglets than in CON-fed piglets. Piglets fed the MB diet had greater villus height and villus height to crypt depth ratio (VC) in the duodenum, villus height in the ileum, and lower crypt depth in the jejunum than did piglets given the CON diet (P < 0.5). Piglets in the MB group had increased concentrations of acetate, propionate, butyrate, and total short-chain fatty acids in the ileum or cecum compared with the CON and AGP groups (P < 0.05). Streptococcus proportion was lower in the MB than in the AGP group. Dietary MB increased the Lactobacillus and decreased Escherichia-Shigella populations compared with the CON group (P < 0.05). The study results indicate that MB can be used to replace AGP as a feed supplement for weaned piglets.

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Simulated gastrointestinal digest from preserved egg white exerts anti-inflammatory effects on Caco-2 cells and a mouse model of DSS-induced colitis

Cited by 42 publications

References 38 publications

Kangfuxin Liquid Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Ulcerative Colitis in Mice by Modulating Immune Response and Suppressing Inflammation

Kangfuxin Liquid Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Ulcerative Colitis in Mice by Modulating Immune Response and Suppressing Inflammation

A comparative study of the modulation of the gut microbiota in rats by dietary intervention with different sources of egg‐white proteins

Effects of Combined Supplementation of Macleaya cordata Extract and Benzoic Acid on the Growth Performance, Immune Responses, Antioxidant Capacity, Intestinal Morphology, and Microbial Composition in Weaned Piglets

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