Simulation and Experiment for Electrode Coverage Evaluation by Electrochemical Impedance Spectroscopy Using Parallel Facing Electrodes

Abstract

Parallel facing electrodes (PFE) for adherent cell monitoring by electrochemical impedance spectroscopy (EIS) was developed, and its characteristics were investigated by both computer simulation and experiment. The PFE consists of two facing gold electrode strips separated by 40 m, and the area of its intersection is 500 m  500 m. Computer simulation of EIS with adherent cells showed distinct difference in solution resistance for different cell coverage, which was confirmed by experimental results using latex beads suspension. A well-defined relationship between solution resistance and cell coverage in our PFE is promising for quantitative evaluation of cell density, morphology and fatality.

An analytical formula for determining the electrical impedance between a single adherent cell and sensor substrate

“…Double-layer capacitance per unit area 46) F m 2 -0.89 Solution relative permittivity 47) 78 Solution conductivity 47) S m 1 -1.5 Cell membrane thickness 46) nm 5.0 Cell membrane relative permittivity 46) 5.0 Cell membrane conductivity 48) S m 1 -1.0 10 9…”