We demonstrate the detection of single hole tunneling through physically defined silicon quantum dots (QDs) by charge sensing. We estimate capacitive couplings between the QDs and tuning gates by simulation based on the Monte Carlo method. In addition, an investigation of spin-related transport is presented. Pauli spin blockade is observed in double QDs, where hole transport is blocked by forbidden transitions between triplet and singlet states. The magnetic field dependence of the leakage current in Pauli spin blockade shows a dip characteristic at zero field, which is explained by spin relaxation due to spin–orbit coupling with phonons. We extract the dip width BC ∼ 65 mT and a spin relaxation rate Γrel ∼ 55 MHz. The small dip width and high spin relaxation rate reflect a strong spin–orbit coupling.