Simultaneous analysis of carotenoids and tocopherols in botanical species using one step solid–liquid extraction followed by high performance liquid chromatography

Valdivielso, Izaskun; Bustamante, María Ángeles; Gordoa, Juan Carlos Ruiz de; Nájera, Ana Isabel; Renobales, Mertxe de; Barrón, Luis Javier R.

doi:10.1016/j.foodchem.2014.10.090

Cited by 24 publications

(17 citation statements)

References 33 publications

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“…α-tocopherol content of Astragalus species have found between 3,99±0,14 µg/g and 11,9±0,66 µg/g, int this study. A-tocopherol was found as main content study by Valdivielso et al [30]. A-tocopherol contents of studied three species in present study were found 1,12±0,17 µg/g and 3,41±0,27 µg/g apart from A. cephalotes var.…”

Section: Discussionsupporting

confidence: 46%

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HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

Bahşi

Kürşat

Emre

et al. 2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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The goal of current study is to contribute the biochemical studies of five Astragalus (Astragalus anthlloides, Astragalus hirsutus, Astragalus campylorhynchus, Astragalus cephalotes var. cephalotes, Astragalus odaratus) species by determining the lipide-soluble vitamin contents based on HPLC analyse. Current study showed that A. anthlloides (208,95±4,09 µg/g) and A. hirsutus (200,9±2,8 µg/g) have high ϒ-tocopherol content. Also, D3 vitamin content of studied Astragalus species were found between 2,94±0,18 µg/g (Astragalus odaratus) and 12,53±,495 µg/g (Astragalus anthlloides). On the other hand, α-tocopherol content of Astragalus species have detected between 3,99±0,14 µg/g (Astragalus campylorhynchus) and 11,9±0,66 µg/g (Astragalus cephalotes var. cephalotes). However, it was found that A. hirsutus only have beta caroten content (3,55±0,43 µg/g) among studied species. On the other hand, it was found that r-tocopherol, a-tocopherol acetate, D2, K1, retinol and retinol acetate contents were lowest amounts or absent.

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Section: Discussionsupporting

confidence: 46%

“…in another study done by E.-Siong et al [28] it was found that carotene content of legumes were 9.2±10 mg/kg. Valdivielso et al [30] indicated that beta-carotene content of legume species were 2.91 ± 0.148 mg/100g. Tocopherols, are lipide-soluble vitamins which play active role the scavenging of free radicals in the cell [31].…”

Section: Discussionmentioning

confidence: 99%

HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

Bahşi

Kürşat

Emre

et al. 2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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“…However, Bağcı et al [45] indicated that α and γ-tocopherol were detected as the most abundant tocopherol components in all of the studied taxa except Lathyrus inconspicuus, Onobrychis luetiana and Onobrychis hypargyrea. It was determined that α-tocopherol acetate was the major compound in all the botanical species except for R. bulbosus in which free α-tocopherol was the major compound work done by Valdivielsa et al [56]. Present study determined that Vicia species have D3 vitamin contents (13,8±0,62 µg/g-50,5±2,13 µg/g).…”

Section: Discussionmentioning

confidence: 53%

The lipid-soluble vitamins contents of some Vicia L. species by using HPLC

Emre

Bahşi

Genç

et al. 2019

International Journal of Secondary Metabolite

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In the present study, lipid-soluble vitamin compositions in the seeds of the Vicia L. taxa (V. ervilia (L.) Willd., V. cuspidata Boiss., V. peregrina L., V. cracca L. subsp. stenophylla Gaudin, V. mollis Boiss.& Hausskn., V. hybrida L., V. sativa L. subsp. nigra (L.) Ehrh. var. nigra L., V. sativa L. subsp. sativa (Ser.) Gaudin var. sativa, V. crocea (Desf.) B. Fedstch., V. noeona Reuter ex Boiss. var. noeona, V. narbonensis L. var. narbonensis) were determined by using HPLC. It was found that studied Vicia species apart from V. ervilia and V. cuspidata have highest ß-carotene contents, 1523,7±6,4 µg/g and 236,62±1,8 µg/g, respectively. Also, this study showed that V. ervilia and V. cuspidata have highest γ-tocopherol content. On the other hand, current study indicated that Vicia species have D3 vitamin contents between 13,8±0,62 µg/g and 50,5±2,13 µg/g. However, the αtocopherol, α-tocopherol acetate, D2, K1, retinol and retinol acetate contents of studied Vicia L. species were lowest.

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“…Fruits can be natural sources of antioxidants such as vitamins (Oliveira et al, 2011;Leong and Oey, 2012), carotenoids (Souza et al, 2014;Valdivielso et al, 2015) and phenolic compounds (Kang et al, 2012;Paz et al, 2015) with antioxidant properties (Castrejón et al, 2008;Koca and Karadeniz, 2009;Kang et al, 2012). The antioxidant activity of these compounds may act at different stages in the oxidation process, lowering free radicals concentration, chelating ions and even decomposing primary products and leading to nonradical compounds (Lobo et al, 2010;Perera et al, 2016) which help balance the immune system (Kang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Optimization of phenolic compounds extraction with antioxidant activity from açaí, blueberry and goji berry using response surface methodology

Moura¹,

Lima²,

Oldoni

et al. 2018

Emir J Food Agric

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The aim of this study was to determine the best extraction conditions of phenolic compounds present in açaí, blueberry and goji berry fruits using the response surface methodology (RSM). The phenolic compounds profile by high-performance liquid chromatography, antioxidant activity was also determined. A factorial 23 design was used to analyze the effect of the solvent (ethanol and water), time (30 and 60 min) and temperature (30 °C and 60 °C) on the extraction of total phenolic compounds (TPC) and activity antioxidant (AA). The variables time and temperature had a positive effect on antioxidant activity (AA) in their highest levels, 60 min and 60 °C, respectively. The solvent ethanol 800 g/mL was more efficient in TPC extracting with AA in all matrices. Rutin was present in high amounts in blueberry and goji berry, and the myricetin in açaí. The açaí showed higher in vitro antioxidant activity when extracted at 60 °C for 60 min. The high correlation coefficient (0.98) of global response (GR) showed that we can find out single and global response in research with multiple dependent variables. The GR analysis indicated the highest values of the TPC and AA when the fruits were extracted at 60°C for 60 min using ethanol as solvent and it was very useful for simplifying and improving the phenolic compounds extraction performance.

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Simultaneous analysis of carotenoids and tocopherols in botanical species using one step solid–liquid extraction followed by high performance liquid chromatography

Cited by 24 publications

References 33 publications

HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

HPLC Kullanarak Bazı Astragalus Türlerinin Yağda Çözünen Vitamin İçerikleri

The lipid-soluble vitamins contents of some Vicia L. species by using HPLC

Optimization of phenolic compounds extraction with antioxidant activity from açaí, blueberry and goji berry using response surface methodology

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