Simultaneous analysis of kasugamycin and validamycin-A in fruits and vegetables using liquid chromatography-tandem mass spectrometry and consecutive solid-phase extraction

Abstract

A method based on a ReproSil 100 C18 column without any ion pairs or buffer additives is proposed for the determination of polar aminoglycosides.

Development of an Analytical Method for Kasugamycin Residue in Herbal Medicine, <i>Achyranthes japonica</i> Nakai

“…Nevertheless, it was reported that the retention time and sensitivity were excellent, and because of confirming this, sufficient retention time and sensitivity were secured, as shown in Fig. 1(c) [7,10,19]. Therefore, in this study, an amide column highly effective for kasugamycin was selected and analyzed.…”

“…Therefore, in this study, water and acetonitrile, commonly used to analyze aminoglycoside antibiotics, were selected as the mobile phase solvent, and 0.1% formic acid solution was added to improve the sensitivity and resolution efficiency of the target component [5,18]. Previous studies found that the sensitivity of the analyte is increased when a 0.1% formic acid solution is added to the mobile phase, while the sensitivity of the signal decreases when a concentration lower than 0.1% is added [8,19].…”

“…As a result of comparing and analyzing the resolution and sensitivity of kasugamycin through C18, HILIC, and amide columns for HPLC analysis, the C18 column was rapidly eluted without staying in the column due to the amino group of kasugamycin, and it was not easy to separate it from the interfering material, as shown in Fig. 1(a) [11,19]. The HILIC column acts effectively on polar basic compounds and aminoglycosides, but its retention time was short, and peak tailing was confirmed, which required improvement, as seen in Fig.…”

Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

“…At present, chromatographic based technology is the mainstream of antibiotic detection, especially high-performance liquid chromatography (HPLC). [11][12][13] Although the sensitivity and accuracy of separation technology based are excellent, the disadvantages such as complicated operations and pretreatment procedures are unavoidable.…”

Paper based microfluidics for rapid preconcentration of micropollutants by ion preconcentration

“…Micropollutants such as heavy metal ions and antibiotic residues are currently studied using solid-liquid extraction, liquid-liquid extraction, and liquid membrane extraction, based on adsorption and extraction fundamentals [12][13][14][15], followed by analytical techniques, such as inductively coupled plasma-mass spectrometry (ICP-MS), mass spectrometry (MS), atomic absorption spectrometry (AAS)-electrothermal, graphite furnace-X-ray fluorescence spectroscopy (R-FS) [16][17][18], liquid chromatography-mass spectrometry (LC-MS), and high-performance liquid chromatography (HPLC) [19][20][21]. These methods are complicated, time-consuming, and expensive and require trained personnel and extensive sample preparation and purification prior to analysis [22,23].…”