Simultaneous analysis of seven alkaloids in Coptis‐Evodia herb couple and Zuojin pill by UPLC with accelerated solvent extraction

Abstract

A rapid, sensitive, and accurate ultra-performance liquid chromatography-UV detection method was developed and validated for simultaneous determination of seven active alkaloids, berberine, palmatine, coptisine, jatrorrhizine, epiberberine, evodiamine, and rutaecarpine, in the Coptis-Evodia herb couple and the Zuojin pill preparation. The original validated method had a total time of about 11 min, which was reduced to 4.7 min by further optimization of gradient elution conditions with eluent A comprising water/methanol/formic acid (v/v/v, 90:10:0.1) and eluent B ACN. An accelerated solvent extraction method was used for the rapid extraction of herb products and pill preparations. Linear behavior over the investigated concentration ranges was observed, with values of r(2) greater than 0.9987 for all analytes. Experiment results were satisfactory for both intra-day and inter-day precision, and method accuracy. Method recovery ranged from 95.7 to 103.5% based on pure reference standards. The validated method was successfully applied to determine amounts of bioactive alkaloid compounds in five samples, in the Coptis-Evodia herb couple, and the commercial Zuojin pill products. The developed method achieved baseline resolution for all seven main basic compounds and is suitable for use as a routine procedure for the rapid identification and quantification of basic compounds in the Coptis-Evodia herb couple and related products.

Anti-ulcerogenic effect of Zuojin Pill against ethanol-induced acute gastric lesion in animal models

“…2). Two compounds were identified and determined: berberine and evodiamine, which is consistent with previous study (Gao et al, 2010). The contents of these compounds in ZJP were observed as: 6.33 mg/g, 1.26 mg/g.…”

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

“…An accelerated solvent extraction (ASE) 100 System (Dionex, Sunnyvale, CA) with 11 mL stainless steel ASE vessels was used for pressurized liquid extraction (Gao et al, 2010). Coptidis Rhizoma, Euodiae Fructus and their products were ground into powders with a particle size 40-60 mesh before use.…”

“…However, in the Pharmacopoeia of the People's Republic of China (Chinese Pharmacopoeia Commission, 2010), berberine is monitored as the signal marker for quality control of Zuojin-related products. Thinlayer chromatography (Shen et al, 1993), capillary electrophoresis (Lee et al, 1997;Wang et al, 2006), HPLC DAD (Chu et al, 1996), LC-MS (Luo et al, 2005), and UPLC-TUV (Gao et al, 2010) were established for analysis of isoquinoline and indolequinazoline alkaloids in this formula for quality control. To date, although, there are a few studies on the determination of alkaloids of the herb couple by HPLC-UV (Chu et al, 1996;Luo et al, 2005), no general fingerprint information is available.…”

In vitro anti-proliferative effects of Zuojinwan on eight kinds of human cancer cell lines

“…The main activity compounds in ZJW are considered to be alkaloids including berberine, coptisine, jateorhizine, palmatine and epiberberine from C. Chinese Frach, and evodiamine and rutaecarpine from E. rutaecarpa (Juss.) Benth (Sheng et al 2006;Yang et al 2009;Gao et al 2010). Pharmacological results have indicated that ZJW has the activites of anti-inflammation, anti-ulcer and anti-acid activities and inhibitory effect on the growth of Helicobacter pylori (Chen et al 2003).…”