Simultaneous Analysis of Streptomycin, Dihydrostreptomycin and Their Related Substances by Capillary Zone Electrophoresis

LC‐MS of streptomycin following desalting of a nonvolatile mobile phase and pH gradient

“…These aberrant sugars are respectively 16 and 14 U higher than SM. The difference of 16 U for the aberrant streptose moiety can be explained by the presence of a hydroxymethyl group at C-49position of the streptose sugar, similar to hydroxystreptomycin described as impurity in SM [6,22]. An extra 14 U in the N-methylglucosamine could be attributed to the presence of N-ethyl or N-dimethyl instead of an N-methyl group at the C-299 position.…”

“…The 162 U can be attributed to a mannosido sugar, so that, peak 9 and 10 were identified as SM B and DHS B, respectively. Both substances are mentioned as possible impurities in SM [10] and DHS [22]. Further confirmation of peak 9 was carried out by injecting a commercial sample containing 10% of SM B.…”

Simultaneous determination of streptomycin and oxytetracycline in agricultural antimicrobials by CZE after an experimental design

“…The running electrolytes recommended in the literature for the determination of OTC by CZE are sodium phosphate (pH 2.0) or sodium carbonate (pH 11.2) [25,26] and for STP are sodium tetraborate (pH 9.0 or 10.25) or sodium dihydrophosphate, boric acid and sodium tetraborate (pH 6.35) [9,11,22]. Thus, in order to optimize the simultaneous determination of STP and OTC different compositions of background electrolyte were evaluated: sodium phosphate (pH 2.0 and 8.5), sodium carbonate (pH 8.5 and 10) and sodium tetraborate (pH 9.0).…”

“…Several studies using capillary electrophoresis for the determination of streptomycin [9,11,22,23,24] or oxytetracycline [4,25,26,27,28,29] in a variety of matrices have been reported, but not for both simultaneously. Hsiao et al [30] proposed a CZE method for the simultaneous determination of STP and OTC, however these authors did not offer a detailed study about the influence of the composition of the electrolyte on the separation of the compounds or indicate the ruggedness of the method.…”

Identification and control of impurities in streptomycin sulfate by high-performance liquid chromatography coupled with mass detection and corona charged-aerosol detection

“…Moreover, a capillary zone electrophoresis (CZE) method with UV detection was reported [11]. However, each of the proposed methods suffers from several drawbacks.…”