Identification and control of impurities in streptomycin sulfate by high-performance liquid chromatography coupled with mass detection and corona charged-aerosol detection

Charged aerosol detection in pharmaceutical analysis

“…Therefore a similar detector response can be expected for different compounds [6,14,[18][19][20]. This is a considerable advantage when screening for impurities where no structural information is available or where the detector response is unknown.…”

“…We [22] found that this system is easy to use and is prone to deliver a unique detector response over an HPLC gradient. Moreover, it could be shown that post column addition of organic modifier to isocratic methods Modified from [19].…”

Challenges in the determination of aminoglycoside antibiotics, a review

“…In case of a sample containing protein, e.g., food, serum and plasma samples, protein precipitation is an initial step. After protein precipitation, sample clean-up to remove fats (if any) by for example Reprinted from [114] with permission …”

“…As the fragment ions with m/z 207 and 189 are frequently used in SRM transitions for the analysis of spectinomycin, these are explained in Figure 7b. MS is primarily used in multiresidue analysis of AGs in food of animal origin [34, 36, 37, 40] and in impurity profiling[112][113][114]. Some recent examples are discussed below.The determination of the AGs hygromycin B, dihydrostreptomycin, amikacin, kanamycin, apramycin, tobramycin, gentamicin and neomycin together with spectinomycin in bovine muscles using IPLC-MS was described[37].…”

“…Monitoring was performed by time-of-flight mass spectrometry (TOF-MS)and confirmed by tandem-quadrupole MS/MS. The LOQs were tested for streptomycin sulfate and streptidine sulfate to be 4.5 µg.mL -1 and 0.4 µg.mL -1 , respectively[114]. A typical chromatogram is shown inFigure 8.…”