Simultaneous capillary GC of acids and sugars as their silyl(oxime) derivatives: Quantitation of chlorogenic acid, raffinose, and pectin substances

Abstract

SummaryOur GC-MS method for the simultaneous quantitation of sugars and acids as their silyl(oxime) derivatives, from one solution by one injection, has been extended to the reproducible determination of high molecular weight compounds sensitive to decomposition yet requiring a high evaporation temperature (e.g. chlorogenic acid and raffinose) and for the quantitation of the decomposition products of pectin (ie., for the determination of galacturonic acid at low ng levels in the presence of a 10-100 fold excess of glucose eluting just before the acid). The optimized GC procedure has been used for quantitation of the sugar and acid (including chlorogenic acid) composition of potato samples, and for the determination of the increasing amount of the decomposition products of pectin substances in apple pulp after different storage times.

Simultaneous quantitation of mono-, di-and trisaccharides by GC-MS of their TMS ether oxime derivatives: II. In honey

“…Honey samples from various Hungarian apiculturists have been indicated by Arabic numbers (1-10): if available, they were also selected by different floral origin, indicated by the same Arabic numbers, such as mixed flower (1)(2)(3)(4)(5)(6)(7)(8), acacia (1)(2)(3)(4)(5)(7)(8)(9), linden (2), sunflower (9), rape (9) and pine (10). Samples were stored in screw capped glass bottles, in a refrigerator, at +5 ~…”

Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

“…Here, we try to evaluate all those parameters which proved to have considerable influence in the quantitation of our target compounds on the basis of their TIC and SFI values. This work is the continuation of recently published papers [2,3,[48][49][50][51] reporting the simultaneous determination of acids and/or sugars as their silyl ester/ether/oxime derivatives, based on their TIC [2,3,49,50], and on their SFI values [2,51].…”

“…However, mass detectors serve simultaneously for qualitative and quantitative analysis in most laboratories, taking advantage of the information of the Mass Spectral Database [1]. More attention should be paid on the quantitative data evaluation possibilities offered by the GC-MS. Several efforts have been made that , involve fragmentation in qualitative terms, and this has been applied mostly to aliphatic carboxylic acids with few exceptions [2,3,24,27,31,[45][46][47]51]. Acids have been investigated, both in their free form [4][5][6][7][8][9][10] and as their methyl [11][12][13][14], pentafluorobenzoyl [14,15], TMS [16][17][18][19][20][21][22][23], and tert.-butyldimethylsilyl (TBDMS) [24][25][26][27] esters.…”

Functions of Bacillus Subtilis BS7.29 in Wasterwater Treatment

“…White water was obtained after 2000 rpm centrifugation for 20 min [12]. 120 mL white water was installed into 500 mL flask, a certain amount of crude enzyme was added, and then the flask was incubated at 50℃, 120 rpm for 2 h. The reaction was terminated with boiling for 5 min prior to subsequent analysis of gas chromatography -mass spectrometry (GC-MS) to determine the qualitative of various components [13,14].…”