Simultaneous detection of 24 oral antidiabetic drugs and their metabolites in urine by liquid chromatography–tandem mass spectrometry

Lam, Y. H.; Leung, Mei Tik; Ching, Chor Kwan; Mak, Tony Wing Lai

doi:10.1016/j.jchromb.2020.122020

Cited by 10 publications

(2 citation statements)

References 12 publications

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“…Because antidiabetic drugs such as repaglinide are used for the treatment of chronic disease, they are continuously released to the surface water systems through industrial and sewage treatment plants at very low concentrations. Several studies have been reported for quantitation of repaglinide, using highly sophisticated, expensive, and sensitive techniques [ 6 – 11 ] due to the low concentrations of repaglinide in aqueous samples. Sample preparation and preconcentration are important to analyze the low concentrations of repaglinide.…”

Section: Introductionmentioning

confidence: 99%

Response surface optimization of a vortex-assisted dispersive liquid–liquid microextraction method for highly sensitive determination of repaglinide in environmental water by HPLC/UV

et al. 2022

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A vortex-assisted dispersive liquid–liquid microextraction (DLLME) method, mated to chemometrics and combined with HPLC/UV detection was optimized and validated for enrichment and determination of repaglinide in environmental samples using nateglinide as an internal standard (IS). A phosphate buffer (10 mM, pH 2.5): acetonitrile (45:55, v/v) was used as a mobile phase with a flow rate of 1 mL/min in an isocratic elution mode. Chemometrics-assisted optimization was performed using a quadratic integrated d-optimal design. The developed model assessed the statistical significance of the independent variables and their interactions to attain the optimum conditions revealing that extractant type, extractant volume and pH are the most influential factors. Optimization of the extraction procedures was performed with the aid of Design Expert 8® software, which suggested 58 different experiments. The optimal conditions were 30 µL of 1-octanol as extractant, 100 µL of acetonitrile as a disperser at pH 8. Under the optimized conditions, the method showed linearity over the range of 1–100 ng/mL with a limit of detection of 0.4 ng/mL. The accuracy, the intra- and inter-day precision were assessed, the %recoveries were found to be between 98.48 and 100.81% with %RSD lower than 1.3. Using chemometrics in method optimization helped achieve the maximum possible enrichment with the least effort, time, and reagents while considering all possible interactions between variables.

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Section: Introductionmentioning

confidence: 99%

Response surface optimization of a vortex-assisted dispersive liquid–liquid microextraction method for highly sensitive determination of repaglinide in environmental water by HPLC/UV

et al. 2022

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“…It has also been determined simultaneously with its degradation products and impurities (Madhavi et al, 2008), with metformin and meglinides class of antidiabatetics (El-Zaher et al, 2018), fourteen synthetic antidiabetics (Cui et al, 2010) by UV detector and HPLC. Estimation of nateglinide in rabbit plasma (Sankalia et al, 2007), rat plasma (Dey et al, 2020) and urine (Lam et al, 2020) has been described. Among these studies, significant peak tailing of nateglinide on stationary phases was reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of Nateglinide in Tablet Formulation by HPLC Using a Pentafluorophenyl Core-Shell Column

Yılmaz¹

2021

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Nateglinide is an insulin secretagogue which has been used for the treatment of Type 2 Diabetes mellitus. A simple analytical methodology for determination of nateglinide in tablet formulation is described. An isocratic reversed phase high performance liquid chromatographic (HPLC) method was developed using Kinetex pentafluorophenyl (PFP) (5µm particle size and 4.6 x 150 mm id) core-shell column as stationary phase and 0.05 M Na2HPO4 (pH=2.0) / methanol (30:70 v/v) mixture as mobile phase with the flow rate of 1.0 mL min -1 and diode-array detector at 215 nm. Column was termostated at 22°C. Under these conditions nateglinide was eluted with a symmetrical peak shape and the retention time was 4.779 minutes. The system suitability parameters such as tailing factor, capacity factor and theoretical plate were evaluted. The method was validated as per International Council on Harmonisation (ICH) guidelines. Linearity was obtained in the 2.54 -40.70 µg mL -1 concentration range with equation y= 10.818x + 4.7048 (R 2 = 0.998). Limit of detection and limit of quantification were 1.07 and 2.54 µg mL -1 . Accuracy of the method was tested by recovery studies which were in the range of 98.78 -100.76 %. The intra-day and inter-day precision studies were carried out and the relative standard deviation values of peak areas were within ICH limits. The proposed method was successfully applied to the determination of nateglinide in tablet dosage form.

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Stability Indicating, pH and pKa Dependent HPLC–DAD Method for the Simultaneous Determination of Weakly Ionizable Empagliflozin, Dapagliflozin and Canagliflozin in Pharmaceutical Formulations

et al. 2020

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Simultaneous detection of 24 oral antidiabetic drugs and their metabolites in urine by liquid chromatography–tandem mass spectrometry

Cited by 10 publications

References 12 publications

Response surface optimization of a vortex-assisted dispersive liquid–liquid microextraction method for highly sensitive determination of repaglinide in environmental water by HPLC/UV

Response surface optimization of a vortex-assisted dispersive liquid–liquid microextraction method for highly sensitive determination of repaglinide in environmental water by HPLC/UV

Determination of Nateglinide in Tablet Formulation by HPLC Using a Pentafluorophenyl Core-Shell Column

Stability Indicating, pH and pKa Dependent HPLC–DAD Method for the Simultaneous Determination of Weakly Ionizable Empagliflozin, Dapagliflozin and Canagliflozin in Pharmaceutical Formulations

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