Simultaneous Detection of Bovine Rotavirus, Bovine Parvovirus, and Bovine Viral Diarrhea Virus Using a Gold Nanoparticle-Assisted PCR Assay With a Dual-Priming Oligonucleotide System

Wang, Mengmeng; Yue, Yan; Wang, Ruichong; Wang, Li; Zhou, Han; Li, Yijing; Tang, Lijie; Jin, Yanping; Cui, Wen; Qiao, Xinyuan

doi:10.3389/fmicb.2019.02884

Cited by 24 publications

(22 citation statements)

References 46 publications

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“…Actually, nanoPCR has been reported to perform multiplex detection of three viruses. 39 Therefore, we believed the developed Taqman-MGB nanoPCR would be a powerful tool for the clinical diagnosis of single-base mutation.…”

Section: Resultsmentioning

confidence: 99%

Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations

Xue¹,

You²,

Peng³

et al. 2021

IJN

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Purpose Detection of single-base mutations is important for real-time monitoring of tumor progression, therapeutic effects, and drug resistance. However, the specific detection of single-base mutations from excessive wild-type background sequences with routine PCR technology remains challenging. Our objective is to develop a simple and highly specific qPCR-based single-base mutation detection method. Methods Using EGRF T790M as a model, gold nanoparticles at different concentrations were separately added into the Taqman-MGB qPCR system to test specificity improvement, leading to the development of the optimal Taqman-MGB nanoPCR system. Then, these optimal conditions were used to test the range of improvement in the specificity of mutant-type and wild-type templates and the detection limit of mutation abundances in a spiked sample. Results The Taqman-MGB nanoPCR was established based on the traditional qPCR, with significantly suppressed background noise and improved specificity for single-base mutation detection. With EGFR T790M as a template, we demonstrated that our Taqman-MGB nanoPCR system could improve specificity across a wide concentration range from 10 −9 μM to 10 μM and detect as low as 0.95% mutation abundance in spiked samples, which is lower than what the traditional Taqman-MGB qPCR and existing PCR methods can detect. Moreover, we also proposed an experimentally validated barrier hypothesis for the mechanism of improved specificity. Conclusion The developed Taqman-MGB nanoPCR system could be a powerful tool for clinical single-base mutation detection.

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Section: Resultsmentioning

confidence: 99%

Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations

Xue¹,

You²,

Peng³

et al. 2021

IJN

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“…AuNPs were then intensively investigated to improve the performance of PCR, and other nanomaterials were also found to amplify the performance [ 34 ]. In this field, AuNPs mainly work as catalysts instead of signal indicators; now, this field has evolved to become nanoPCR [ 35 , 36 , 37 , 38 ]. However, the mechanism remains elusive, which is mainly explained by: AuNPs absorbing polymerase to modulate the amount of active polymerase; AuNPs absorbing primer to decrease the T m of primers; and AuNPs absorbing the PCR product to speed up product association [ 37 , 39 , 40 , 41 , 42 ].…”

Section: Non-specific Combination Of Aunps With Conventional Pcr Productmentioning

confidence: 99%

The Integration of Gold Nanoparticles with Polymerase Chain Reaction for Constructing Colorimetric Sensing Platforms for Detection of Health-Related DNA and Proteins

Wang

Liu

et al. 2022

Biosensors

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Polymerase chain reaction (PCR) is the standard tool in genetic information analysis, and the desirable detection merits of PCR have been extended to disease-related protein analysis. Recently, the combination of PCR and gold nanoparticles (AuNPs) to construct colorimetric sensing platforms has received considerable attention due to its high sensitivity, visual detection, capability for on-site detection, and low cost. However, it lacks a related review to summarize and discuss the advances in this area. This perspective gives an overview of established methods based on the combination of PCR and AuNPs for the visual detection of health-related DNA and proteins. Moreover, this work also addresses the future trends and perspectives for PCR–AuNP hybrid biosensors.

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“…It can be caused by noninfectious factors such as environmental stress, management practices, and nutritional issues or by various infectious agents including bacteria such as Salmonella spp., Clostridium perfringens, and Escherichia coli, viruses such as bovine coronavirus (BCV), bovine rotavirus (BRV), and bovine viral diarrhea virus, and protozoa such as Coccidia and Cryptosporidium [3,4]. In particular, BRV is a major cause of neonatal calf diarrhea in calves aged 15-45 days [5,6], who can shed a high viral load through their feces, meaning that widespread infection is possible from even minimal environmental contamination [7]. Therefore, it is important to identify and manage infected groups via effective virus detection to prevent proliferation.…”

Section: Introductionmentioning

confidence: 99%

Affinity Peptide-based Electrochemical Biosensor for the Highly Sensitive Detection of Bovine Rotavirus

Cho

Park

2022

Biotechnol Bioproc E

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Bovine diarrhea is a major concern in the global bovine industry because it can cause significant financial damage. Of the many potential infectious agents that can lead to bovine diarrhea, bovine rotavirus (BRV) is a particular problem due to its high transmissibility and infectivity. Therefore, it is important to prevent the proliferation of BRV using an early detection system. This study developed an affinity peptide-based electrochemical method for use as a rapid detection system for BRV. A BRV-specific peptide was identified via the phage display technique and chemically synthesized. The synthetic peptide was immobilized on a gold electrode through thiol-gold interactions. The performance of the BRV specific binding peptides was evaluated using square wave voltammetry. The developed detection system exhibited a low detection limit (5 copies/mL) and limit of quantitation (2.14 × 10 2 copies/mL), indicating that it is a promising sensor platform for the monitoring of BRV.

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Simultaneous Detection of Bovine Rotavirus, Bovine Parvovirus, and Bovine Viral Diarrhea Virus Using a Gold Nanoparticle-Assisted PCR Assay With a Dual-Priming Oligonucleotide System

Cited by 24 publications

References 46 publications

Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations

Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations

The Integration of Gold Nanoparticles with Polymerase Chain Reaction for Constructing Colorimetric Sensing Platforms for Detection of Health-Related DNA and Proteins

Affinity Peptide-based Electrochemical Biosensor for the Highly Sensitive Detection of Bovine Rotavirus

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