Simultaneous detection of pathogenic Listeria including atypical Listeria innocua in vegetables by a quadruplex PCR method

Rosimin, Aurelius Albert; Kim, Min-Ju; Joo, In-Sun; Suh, Soo-Hwan; Kim, Keun-Sung

doi:10.1016/j.lwt.2016.02.007

Cited by 15 publications

(11 citation statements)

References 34 publications

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“…Listeria innocua was chosen to investigate the antilisterial action expressed by PLA, considering that this species is regarded as a non-pathogenic indicator for the presence of L. monocytogenes in foods and the high similarity between the two species ( Rosimin et al, 2016 ; Tremonte et al, 2016b ).…”

Section: Resultsmentioning

confidence: 99%

Detection of Antilisterial Activity of 3-Phenyllactic Acid Using Listeria innocua as a Model

et al. 2018

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The 3-Phenyllactic acid (PLA) produced by various lactic acid bacteria (LAB) possesses a broad spectrum of antimicrobial activity. In this study, the effect of PLA against Listeria innocua was studied with the aim to obtain additional information about its mechanism of action. The effect of pH on the antilisterial activity of PLA was investigated and a pH-dependent behavior, typical of weak acid, was detected. The antilisterial effect of PLA was firstly compared to that produced by lactic acid (LA) and than to that expressed by phenolic acids (gallic, caffeic, and ferulic acids) evaluating minimum inhibitory concentration (MIC), MBC, and survival kinetic parameters. PLA showed MIC values and death kinetic parameters significantly different from those exhibited by LA and by tested phenolic acids. In particular, the MIC value observed for PLA vs L. innocua resulted lower than that of the other preservative compounds studied herein, and consistent with the quantity generally produced by LAB. Moreover, the effect of PLA and phenolic acids on bacterial surface charge and loss of cellular content resulted different. The overall results highlighted strong differences in the antilisterial mechanism of action among PLA and other compounds such as LA and phenols. Specifically, it is possible to hypothesize that the antilisterial mechanism of action due to PLA is associated with the affinity to cell surface, which contributes to the cellular damage.

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Section: Resultsmentioning

confidence: 99%

Detection of Antilisterial Activity of 3-Phenyllactic Acid Using Listeria innocua as a Model

et al. 2018

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“…Listeria innocua is regarded as a non-pathogenic indicator for the presence of Listeria monocytogenes in foods (Rosimin et al, 2016). Numerous ecological and genomic comparative studies highlighted a high similarity between the two species (Glaser et al, 2001; Girardin et al, 2005; den Bakker et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Homology-Based Modeling of Universal Stress Protein from Listeria innocua Up-Regulated under Acid Stress Conditions

et al. 2016

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An Universal Stress Protein (USP) expressed under acid stress condition by Listeria innocua ATCC 33090 was investigated. The USP was up-regulated not only in the stationary phase but also during the exponential growth phase. The three dimensional (3D) structure of USP was predicted using a combined proteomic and bioinformatics approach. Phylogenetic analysis showed that the USP from Listeria detected in our study was distant from the USPs of other bacteria (such as Pseudomonas spp., Escherichia coli, Salmonella spp.) and clustered in a separate and heterogeneous class including several USPs from Listeria spp. and Lactobacillus spp. An important information on the studied USP was obtained from the 3D-structure established through the homology modeling procedure. In detail, the Model_USP-691 suggested that the investigated USP had a homo-tetrameric quaternary structure. Each monomer presented an architecture analogous to the Rossmann-like α/β-fold with five parallel β-strands, and four α-helices. The analysis of monomer-monomer interfaces and quality of the structure alignments confirmed the model reliability. In fact, the structurally and sequentially conserved hydrophobic residues of the β-strand 5 (in particular the residues V146 and V148) were involved in the inter-chains contact. Moreover, the highly conserved residues I139 and H141 in the region α4 were involved in the dimer association and functioned as hot spots into monomer–monomer interface assembly. The hypothetical assembly of dimers was also supported by the large interface area and by the negative value of solvation free energy gain upon interface interaction. Finally, the structurally conserved ATP-binding motif G-2X-G-9X-G(S/T-N) suggested for a putative role of ATP in stabilizing the tetrameric assembly of the USP. Therefore, the results obtained from a multiple approach, consisting in the application of kinetic, proteomic, phylogenetic and modeling analyses, suggest that Listeria USP could be considered a new type of ATP-binding USP involved in the response to acid stress condition during the exponential growth phase.

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“…milk intentionally added with L. innocua strains and stored at 8°C for 72 hr. L. innocua was chosen because it is regarded as a non‐pathogenic indicator for the presence of L. monocytogenes in foods (Rosimin, Kim, Joo, Suh, & Kim, ; Tremonte, Succi, et al, ). In our study, the antimicrobial activity of boiling and microwave treatment was investigated on multiple strains of L. innocua inoculated at an initial dose of about 4 log cfu/ml in raw milk.…”

Section: Resultsmentioning

confidence: 99%

Concerns and solutions for raw milk from vending machines

Pannella

Messia

Tremonte

et al. 2019

J Food Process Preserv

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The sale of raw milk from vending machines is allowed in several European Countries. Since unpasteurized milk could harbor foodborne pathogens, the boiling treatment is highly recommended before consumption. In this study, the effect of storage temperatures, generally recorded in domestic refrigerators, as well as the effect of domestic boiling and industrial microwaving on the microbiological, and nutritional quality of raw bovine milk from vending machines was evaluated. Results highlighted that some microbial groups increased during the storage at 8°C. Noticeable, Listeria spp., even if initially present in low amounts, reached levels that would be a cause of concerns. Based on these results, the effects of domestic boiling and microwaving on the survival of L. innocua intentionally added was evaluated, as well as the nutritional features of treated milk. Both treatments were able to eliminate L. innocua, but only microwaving preserved the nutritional quality of milk. Practical applications This study presents the application of microwaving as an alternative treatment to enhance the quality of raw bovine milk. Domestic storage conditions (especially refrigeration temperatures and time) produce a strong decay in the microbiological quality of the product and Listeria levels might become a real concern. In addition, boiling treatment, imposed by law prior to milk consumption, produces a further depletion of the nutritional quality, producing a strong decay in thermolabile compounds. A suitable solution for heat treatment of raw milk from vending machines could be represented by microwaving at 780 W for 1.35 min. This datum could serve for setting a specific program in domestic microwave ovens for sanitization of raw milk in home environments.

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Simultaneous detection of pathogenic Listeria including atypical Listeria innocua in vegetables by a quadruplex PCR method

Cited by 15 publications

References 34 publications

Detection of Antilisterial Activity of 3-Phenyllactic Acid Using Listeria innocua as a Model

Detection of Antilisterial Activity of 3-Phenyllactic Acid Using Listeria innocua as a Model

Homology-Based Modeling of Universal Stress Protein from Listeria innocua Up-Regulated under Acid Stress Conditions

Concerns and solutions for raw milk from vending machines

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