The growth of the chemical industry has brought tremendous challenges to chemical sensing technology. Chemical sensors based on metamaterials have great potential because of their label-free and non-destructive characteristics. However, metamaterials applied in chemical sensing have mainly been investigated from the measurement of sample concentration or the determination of the dielectric properties at a fixed frequency. Here we present a metamaterial integrated microfluidic (MIM) sensor for the multi-band sensing for dielectric property of chemicals, which is promising for the identification of chemicals. The MIM sensor mainly consists of multiple pair of high sensitive symmetrical double split-ring resonators (DSRRs) and meandering microfluidic channels with a capacity of only 4 μL. A dielectric model has been innovatively established and experimentally verified to accurately estimate the complex permittivity and thus realize the multi-band sensing of dielectric property of chemicals. With the increase in the number of resonators in the sensor, a dielectric spectrum like curve could be obtained for more detailed dielectric information. This work delivers a miniaturized, reusable, label-free and non-destructive metamaterial-microfluidic solution and paves a way of the multi-band sensing for dielectric property of chemicals.