Simultaneous determination of 10 kinds of biogenic amines in rat plasma using high‐performance liquid chromatography coupled with fluorescence detection

Abstract

We describe a simple, rapid, selective and sensitive HPLC method coupled with fluorescence detection for simultaneous determination of 10 kinds of biogenic amines (BAs: tryptamine, 2-phenethylamine, putrescine, cadaverine, histamine, 5-hydroxytryptamine, tyramine, spermidine, dopamine and spermine). BAs and IS were derivated with dansyl chloride. Fluorescence detection (λ /λ = 340/510 nm) was used. A satisfactory result for method validation was obtained. The assay was shown to be linear over the ranges 0.005-1.0 μg/mL for tryptamine, 2-phenethylamine and spermidine, 0.025-1.0 μg/mL for putrescine, 0.001-1.0 μg/mL for cadaverine, 0.25-20 μg/mL for histamine, 0.25-10 μg/mL for 5-hydroxytryptamine and dopamine, and 0.01-1.0 μg/mL for tyramine and spermine. The limits of detection and the limits of quantification were 0.3-75.0 ng/mL and 1.0-250.0 ng/mL, respectively. Relative standard deviations were ≤5.14% for intra-day and ≤6.58% for inter-day precision. The recoveries of BAs ranged from 79.11 to 114.26% after spiking standard solutions of BAs into a sample at three levels. Seven kinds of BAs were found in rat plasma, and the mean values of tryptamine, 2-phenethylamine, putrescine, cadaverine, histamine, spermidine and spermine determined were 52.72 ± 7.34, 11.45 ± 1.56, 162.56 ± 6.26, 312.75 ± 18.11, 1306.50 ± 116.16, 273.89 ± 26.41 and 41.51 ± 2.07 ng/mL, respectively.

Study of Histamine Detection using Liquid Chromatography and Gas Chromatography

“…al., 2016;Comas -Baste et. al., 2017;Wang et al, 2018). Generally, a derivatization step with a chromophore or fluorophore is required to increase the sensitivity and selectivity during analysis.…”

Current Trends in Detection of Histamine in Food and Beverages

“…Following fluorescence detection, a novel method allowing diagnosis of histamine intolerance by histamine and methylhistamine detection in urine by ultrahigh performance liquid chromatography coupled with fluorimetric detector (UHPLC-FL) was developed in 2017 . An HPLC method from 2018 with fluorescence detection allowed the determination of histamine together with nine other biogenic amines after derivatization with dansyl chloride with linearity in the range 0.25–10 μg/mL and a detection limit of 75 ng/mL for histamine in rat plasma . Coupling HPLC or GC with mass detection allows a decrease in the detection limit of histamine even more.…”

Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

“…Pharmaceutical molecules containing nitrogen based functional groups like primary and secondary amines, imidazoles and also other groups like phenols were derivatized effectively with dansyl chloride and quantified accurately in biological and pharmaceutical preparations. [22][23][24][25][26][27][28]. The purpose of the current study is to demonstrate the efficiency of DNS-Cl as an suitable fluorescent labelling reagent for the new, selective and sensitive determination of TCG in pharmaceutical preparation and spiked human plasma.…”