Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

Abstract

A sensitive method is presented for the determination of the ticagrelor (TCG) in human plasma and pharmaceutical preparations using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride). Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate (pH 10) to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0-200.0 ng mL −1 , with a coefficient of determination of 0.9999 with a limit of detection (LOD) of 0.14 ng mL −1 and the limit of quantitation (LOQ) of 0.46 ng mL −1. The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.

Sample pretreatment and HPLC determination of antiplatelet drug ticagrelor in blood plasma from patients with acute coronary syndromes

“…One square wave voltammetric method (Rizk et al, 2017) and two fluorimetric methods have been developed for the determination of TCG in biological samples. A fluorimetric method based on TCG natural fluorescence was used only for plasma analysis in rat (Elmansi et al, 2020), whereas fluorescence derivatization was applied to spiked human plasma (Önal & Kepekçi Tekkeli, 2020). A fluorine nuclear magnetic resonance spectroscopy method (Zhang et al, 2021) is also in the development stage but cannot be applied to real biological samples currently.…”