Simultaneous determination of 5-hydroxyindoles and catechols by high-performance liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine

Fujino, Kaoru; Yoshitake, Takashi; Kehr, Ján; Nohta, Hitoshi; Yamaguchi, Masatoshi

doi:10.1016/s0021-9673(03)01180-4

Cited by 71 publications

(48 citation statements)

References 26 publications

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“…After addition of the internal standard (3,4-dihydroxynorephedrine), plasma NE was purified using the diphenylborate extraction method. The extracted NE was reacted with benzylamine to form a fluorescent derivative (14,43,47,48). NE and the internal standard derivatives were separated on HPLC using a reverse-phase column (XBridge C-18, Waters) and the Alliance HPLC system equipped with a fluorescence detector (model 2475, Waters).…”

Section: Methodsmentioning

confidence: 99%

Prolonged infusion of amino acids increases leucine oxidation in fetal sheep

Maliszewski

Gadhia

O'Meara

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Maliszewski AM, Gadhia MM, O'Meara MC, Thorn SR, Rozance PJ, Brown LD. Prolonged infusion of amino acids increases leucine oxidation in fetal sheep. Am J Physiol Endocrinol Metab 302: E1483-E1492, 2012. First published March 27, 2012 doi:10.1152/ajpendo.00026.2012.-Maternal high-protein supplements designed to increase birth weight have not been successful. We recently showed that maternal amino acid infusion into pregnant sheep resulted in competitive inhibition of amino acid transport across the placenta and did not increase fetal protein accretion rates. To bypass placental transport, singleton fetal sheep were intravenously infused with an amino acid mixture (AA, n ϭ 8) or saline [control (Con), n ϭ 10] for ϳ12 days during late gestation. Fetal leucine oxidation rate increased in the AA group (3.1 Ϯ 0.5 vs. 1.4 Ϯ 0.6 mol·min Ϫ1 ·kg Ϫ1 , P Ͻ 0.05). Fetal protein accretion (2.6 Ϯ 0.5 and 2.2 Ϯ 0.6 mol·min Ϫ1 ·kg Ϫ1 in AA and Con, respectively), synthesis (6.2 Ϯ 0.8 and 7.0 Ϯ 0.9 mol·min Ϫ1 ·kg Ϫ1 in AA and Con, respectively), and degradation (3.6 Ϯ 0.6 and 4.5 Ϯ 1.0 mol·min Ϫ1 ·kg Ϫ1in AA and Con, respectively) rates were similar between groups. Net fetal glucose uptake decreased in the AA group (2.8 Ϯ 0.4 vs. 3.9 Ϯ 0.1 mg·kg Ϫ1 ·min Ϫ1 , P Ͻ 0.05). The glucose-O2 quotient also decreased over time in the AA group (P Ͻ 0.05). Fetal insulin and IGF-I concentrations did not change. Fetal glucagon increased in the AA group (119 Ϯ 24 vs. 59 Ϯ 9 pg/ml, P Ͻ 0.05), and norepinephrine (NE) also tended to increase in the AA group (785 Ϯ 181 vs. 419 Ϯ 76 pg/ml, P ϭ 0.06). Net fetal glucose uptake rates were inversely proportional to fetal glucagon (r 2 ϭ 0.38, P Ͻ 0.05), cortisol (r 2 ϭ 0.31, P Ͻ 0.05), and NE (r 2 ϭ 0.59, P Ͻ 0.05) concentrations. Expressions of components in the mammalian target of rapamycin signaling pathway in fetal skeletal muscle were similar between groups. In summary, prolonged infusion of amino acids directly into normally growing fetal sheep increased leucine oxidation. Amino acid-stimulated increases in fetal glucagon, cortisol, and NE may contribute to a shift in substrate oxidation by the fetus from glucose to amino acids. pregnancy; insulin; fetal protein metabolism; fetal growth PREVIOUS ATTEMPTS TO IMPROVE fetal outcomes with use of maternal high-protein supplementation have actually increased the risk for small-for-gestational-age birth and infant mortality (26,36). Similarly, supplementation of pregnant sheep with intravenous amino acids for several days at the end of gestation demonstrated adverse fetal effects, including hypoxia and respiratory and metabolic acidosis (34). The mechanisms that explain poor fetal outcomes in response to maternal protein supplementation during pregnancy are unknown.Experiments using normal pregnant sheep have led to some testable explanations for how high-protein supplementation during pregnancy might adversely affect fetal outcomes (4). Short-and long-term maternal amino acid infusions using mixtures of amino acids with a higher essential-nonessential ...

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Section: Methodsmentioning

confidence: 99%

Prolonged infusion of amino acids increases leucine oxidation in fetal sheep

Maliszewski

Gadhia

O'Meara

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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“…14) Moreover, the HPLC-FLD method cannot determine 3-MT and HVA due to failure of the derivatization with fluorescent moiety. 3,14) In contrast to the previously reported methods, this derivatization method with ethyl chloroformate using the HPLC-MS system offers the advantages of less time required for the derivatization process and the ability to be performed in aqueous media. 33) In addition, this derivatization demonstrated satisfactory derivatization efficiencies with good yield in a short reaction time and enhanced data collection 34) because not only the levels of 5-HT, DA and their metabolites were simultaneously determined, but each peak area of 5-HT, DA and their metabolites was significantly expanded and more sensitive than those generated when using the HPLC-ECD method.…”

Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 99%

“…2,3) PD is pathologically characterized by the death of dopaminergic neurons in the substantia nigra pars compacta 4) and caused by neurotoxins such as 1-methyl-4-phenyl-1,2,3,6-tetrahypyridine (MPTP), which produces a clinical syndrome similar to PD. Because the administration of MPTP is known to affect the dopaminergic neurons in the nigrostriatal system with subsequent depletion of DA and its metabolites, 5,6) the measurement of 5-HT, DA and their metabolites is necessary to discover and test new drugs for treating these disorders.…”

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confidence: 99%

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Park

Myung

Kim

et al. 2013

Biological & Pharmaceutical Bulletin

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In order to measure the levels of serotonin (5-hydroxyltryptamine, 5-HT), dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) simultaneously, an effective derivatization method followed by high-performance liquid chromatography (HPLC) coupled to electrochemical ionization mass spectrometry was used. The derivatization reaction of biological samples with ethyl chloroformate occurred rapidly at room temperature in aqueous conditions, and the resulting derivatives were isocratically separated with good selectivity using a C18 reversed-phase column within 30 min. The study results showed that the new derivatization procedure offers an excellent means of simultaneous determination of 5-HT, DA and their metabolites in mouse brain homogenates, which are important in a number of physiological and behavioral functions.Key words neurotransmitter; HPLC-MS; ethyl chloroformate; derivatization Dopamine (DA) and serotonin (5-hydroxyltryptamine, 5-HT), which are widely distributed as a monoamine neurotransmitter in the central nervous system (CNS), 1) play important physiological and behavioral roles such as the control and regulation of affective behavior, sleep, reward system, motor and cognitive functions.2) Changes of DA and 5-HT basal levels have been linked to some CNS-related disorders such as depression, panic disorder, schizophrenia and Parkinson's disease (PD).2,3) PD is pathologically characterized by the death of dopaminergic neurons in the substantia nigra pars compacta 4) and caused by neurotoxins such as 1-methyl-4-phenyl-1,2,3,6-tetrahypyridine (MPTP), which produces a clinical syndrome similar to PD. Because the administration of MPTP is known to affect the dopaminergic neurons in the nigrostriatal system with subsequent depletion of DA and its metabolites, 5,6) the measurement of 5-HT, DA and their metabolites is necessary to discover and test new drugs for treating these disorders. 7)Various analytical methods have been widely investigated for determining the level of monoamine neurotransmitters and their metabolites, including high-performance liquid chromatography (HPLC) coupled with electrochemical detection (ECD), dual ECD, fluorescence detection (FLD), FLD/ECD system, chemiluminescence detection and mass spectrometer (MS) and gas chromatography (GC) coupled with MS in biological samples such as plasma, rat brain, and mouse brain and in vitro experiments.8-10) HPLC-ECD, which is the most common analytical technique used to determine monoamines and their metabolites, has the advantages of low cost and fast speed 11) but maintaining its routine use is problematic due to sudden noise problems.12) The HPLC-FLD method is used to measure fluorescent derivatives of monoamine neurotransmitters by a derivatization procedure, 13) which was performed by benzylamine and 1,2-diphenylethylenediamine in a two-step reaction in weakly alkaline media and in the presence of potassium hexacyanoferrate (III).14) However, since this protocol requires the vigorous reaction ...

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“…So far several analytical methods such as capillary electrochromatography, 2 chromatography, 3 and spectrophotometry 4 have been developed to analyze HQ. However, most of these methods have some disadvantages such as complicated pretreatment, time-consuming, low sensitivity and high cost.…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of Graphene-Zn/Al Layered Double Hydroxide (LDH) Composite for Selective Determination of Hydroquinone

Kwon

Hong²

2013

Bulletin of the Korean Chemical Society

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A graphene-Zn/Al layered double hydroxide composite film was simultaneously prepared by electrochemical deposition on the surface of a glassy carbon electrode (G-LDH/GCE) from the mixture solution containing GO and nitrate salts of Zn 2+ and Al 3+. The modified electrode showed good electrochemical performances toward the simultaneous electrochemical detection of hydroquinone (HQ), catechol (CA) and resorcinol (RE) due to the unique properties of graphene (G) and LDH such as large active surface area, facile electronic transport and high electrocatalytic activity. The redox characteristics of G-LDH/GCE were investigated with cyclic voltammetry and differential pulse voltammetry. The well-separated oxidation peak potentials, corresponding to the oxidation of HQ, CA and RE, were observed at 0.126 V, 0.228 V and 0.620 V respectively. The amperometric response of the modified electrode exhibited that HQ can be detected without interference of CA and RE. Under the optimized conditions, the oxidation peak current of HQ is linear with the concentration of HQ from 6.0 µM to 325.0 µM with the detection limit of 0.077 µM (S/N=3). The modified electrode was successfully applied to the direct determination of HQ in a local tap water, showing reliable recovery data.

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Simultaneous determination of 5-hydroxyindoles and catechols by high-performance liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine

Cited by 71 publications

References 26 publications

Prolonged infusion of amino acids increases leucine oxidation in fetal sheep

Prolonged infusion of amino acids increases leucine oxidation in fetal sheep

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Electrodeposition of Graphene-Zn/Al Layered Double Hydroxide (LDH) Composite for Selective Determination of Hydroquinone

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