Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry

Kim, Thomas; London, Amy; Kharasch, Evan D.

doi:10.1016/j.jpba.2011.01.040

Cited by 9 publications

(2 citation statements)

References 26 publications

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“…Analytical methods . Plasma MDZ and ALF concentrations were measured by high‐performance liquid chromatograph tandem mass spectrometry with multiple reaction monitoring as previously reported 48 . Interday coefficients of variation were 7, 8, and 7% at 1.4, 12, and 100 ng/ml MDZ, and 9, 7, and 7% at 1, 10, and 100 ng/ml ALF, respectively.…”

Section: Methodsmentioning

confidence: 97%

Sensitivity of Intravenous and Oral Alfentanil and Pupillary Miosis as Minimal and Noninvasive Probes for Hepatic and First-Pass CYP3A Induction

Kharasch

Francis

London

et al. 2011

Clin Pharmacol Ther

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Systemic and oral clearances of alfentanil are in vivo probes for hepatic and first-pass CYP3A. Both alfentanil single point plasma concentrations and miosis are surrogates for area under the concentration-time curve (AUC), clearance, and are minimal and non-invasive CYP3A probes. This investigation determined alfentanil sensitivity for detecting graded CYP3A induction, and compared it with that of midazolam Twelve volunteers (sequential crossover) received 0, 5, 10, 25 or 75 mg oral rifampin for 5d. Midazolam and alfentanil were given intravenously and orally on sequential days. Dark-adapted pupil diameters were measured with blood sampling. Graded rifampin decreased plasma midazolam AUCs to 83, 76, 62 and 59% (intravenous) and 78, 66, 39, and 24% (oral) of control. Hepatic and first-pass CYP3A induction were detected comparably by plasma midazolam and alfentanil AUCs. Single alfentanil concentrations detected all CYP3A induction, while midazolam was less sensitive. Alfentanil miosis detected induction of first-pass but not hepatic CYP3A.

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Section: Methodsmentioning

confidence: 97%

Sensitivity of Intravenous and Oral Alfentanil and Pupillary Miosis as Minimal and Noninvasive Probes for Hepatic and First-Pass CYP3A Induction

Kharasch

Francis

London

et al. 2011

Clin Pharmacol Ther

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“…HPLC with UV/Vis detection has been used for MDZ determination with an LOD in the range of 0.2–22.7 ng/mL and plasma requirement per assay in the range of 0.2–2 mL . The LOD can be reduced by an order of magnitude (∼0.01 ng/mL) by using HPLC with MS detection or GC with MS detection , using about the same plasma volume per assay (0.1–1.0 mL). GC with electron capture detector (ECD) or nitrogen phosphorus detection (NPD) made it possible to attain an LOD in the range 0.5–3 ng/mL, using plasma volumes of about 1 mL per assay, which are comparable with those used for HPLC–UV/Vis.…”

Section: Introductionmentioning

confidence: 99%

Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Jaček

Matějčková

Málek

et al. 2013

J of Separation Science

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GC with nitrogen phosphorus detection and HPLC with UV detection were used to determine midazolam (MDZ) levels in rabbit plasma following ocular and nasal administration. For GC with nitrogen phosphorus detection, the analyte was extracted from the plasma using a three-step liquid-liquid extraction including extraction with an isopropanol/butyl chloride mixture in an alkaline solution, followed by extractions with 1 M HCl, and finally with an alkaline solution of butyl chloride. The recovery of MDZ was dependent on the sample alkalization time prior to the final extraction. The procedure increased the recovery of MDZ up to 99.6%. Improved sample preparation led to a significant increase in the sensitivity of the determination by GC with nitrogen phosphorus detection. The achieved detection limit was 0.34 ng/mL, which is ten times lower than that obtained using HPLC with UV detection. The small plasma volume was another advantage of the GC with nitrogen phosphorus detection method (200 μL per assay). Both administration routes of the anesthetic (nasal and ocular) resulted in comparable plasma MDZ levels. Kinetic simulation of the MDZ plasma was performed for both administration routes.

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Determination of four antiepileptic drugs in plasma using ultra‐performance liquid chromatography with mass detection technique

Hassib

Hashem

Mahrouse

et al. 2018

Biomedical Chromatography

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Status epilepticus (SE) is considered the second most frequent neurological emergency. Its therapeutic management is performed using sequential antiepileptic drug regimens. Diazepam (DIA), midazolam (MID), phenytoin (PHT) and phenobarbital (PB) are four drugs of different classes used sequentially in the management of SE. A sensitive, selective, accurate and precise method was developed and validated for simultaneous determination of the four antiepileptic drugs in human plasma. Their separation and quantification were achieved using ultra-performance liquid chromatography (UPLC) with mass detection using carbamazepine as internal standard (IS). For the first three drugs and the IS, UPLC-MS/MS with electrospray ionization working in multiple reaction monitoring mode was used at the following transitions: m/z 285 → 193 for DIA; m/z 326 → 291 for MID; m/z 253 → 182 for PHT; and m/z 237 → 194, 237 → 192 for IS. For the fourth drug (PB), a molecular ion peak of PB [M + H] at m/z 233 was used for its quantitation. The method was linear over concentration ranges 5-500 ng/mL for DIA and MID and 0.25-20 μg/mL for PHT and PB. Bioanalytical validation of the developed method was carried out according to European Medicines Agency guidelines. The developed method can be applied for routine drug analysis, therapeutic drug monitoring and bioequivalence studies.

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Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry

Cited by 9 publications

References 26 publications

Sensitivity of Intravenous and Oral Alfentanil and Pupillary Miosis as Minimal and Noninvasive Probes for Hepatic and First-Pass CYP3A Induction

Sensitivity of Intravenous and Oral Alfentanil and Pupillary Miosis as Minimal and Noninvasive Probes for Hepatic and First-Pass CYP3A Induction

Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Determination of four antiepileptic drugs in plasma using ultra‐performance liquid chromatography with mass detection technique

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