Simultaneous determination of amoxicillin and clavulanic acid in human plasma by isocratic reversed-phase HPLC using UV detection

Foroutan, Seyed Mohsen; Zarghi, Afshin; Shafaati, Alireza; Khoddam, Arash; Movahed, Hooman

doi:10.1016/j.jpba.2007.06.019

Cited by 67 publications

(39 citation statements)

References 16 publications

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“…There are several methods known in the literature for the individual and/or simultaneous determination of amoxicillin and clavulanic acid in human plasma and other biological matrix using UV [2,6,11,13] and MS/MS methods [3][4][5][7][8][9]12]. All MS/MS methods involve polarity switching, for monitoring the clavulanic acid in negative electrospray ionization mode.…”

Section: Determination Of Acquisition Parametersmentioning

confidence: 99%

Validated LC-MS/MS Method for the Concomitant Determination of Amoxicillin and Clavulanic Acid from Human Plasma

Balázsi¹,

Paizs²,

Irimie³

et al. 2017

Studia UBB Chemia

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ABSTRACT. The purpose of this study was the development and validation of an LC-MS/MS method, for the concomitant and rapid determination amoxicillin and clavulanic acid from human plasma. The sample workup involved a simple protein precipitation procedure. A core/shell type analytical column (50×2,1 mm, 2.6 Å) was used with PFP stationary phase. A mobile phase with high aqueous composition provided satisfactory separation with good accuracy and precision (stable ionization). The mass spectrometer was operated in positive electrospray ionization mode for both analytes and internal standard. The following parameters were evaluated for validation purpose: Selectivity, sensitivity, matrix effect, anticoagulant effect, linearity, precision and accuracy, recovery, analyte/IS stability in solvent/matrix and carryover. The validated calibration range was 190-22222 ng/ml for amoxicillin, and 147-4908 ng/ml for clavulanic acid. The correlation coefficient R 2 was at least 0.99 for both analytes. The validated method has been successfully used for the evaluation bioequivalence of generic amoxicillin/potassium clavulanate formulations.

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Section: Determination Of Acquisition Parametersmentioning

confidence: 99%

Validated LC-MS/MS Method for the Concomitant Determination of Amoxicillin and Clavulanic Acid from Human Plasma

Balázsi¹,

Paizs²,

Irimie³

et al. 2017

Studia UBB Chemia

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“…Immediately after each blood collection, the samples were centrifuged at 3000 × g for 15 min and plasma was then separated and stored at "70°C. The stored samples were then studied by HPLC in appropriate method [19][20] .…”

Section: Blood Sample Collectionmentioning

confidence: 99%

“…Though there were different methods to estimate amoxicillin in plasma [19][20][21][22][23] , however, none was executed in Bangladesh and therefore the initial challenge was to establish a suitable method in laboratories of Bangladesh. Therefore, the aim of this study was to establish a method of estimation of amoxicillin as well as to get preliminary idea about the pharmacokinetic behavior of Bangladeshi healthy volunteers of two different ethnic origins.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic parameters of Amoxicillin in Bangladeshi volunteers: a preliminary evaluation

Chowdhury

Islam

Rahman

2014

Bangladesh J Physiol Pharmacol

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“…For this reason, the phenyl column was selected for further analysis. 12 Several urine blanks from males and females were injected directly into a micellar chromatographic system in order to check the background. The profile of the protein band and the endogenous compounds for all the samples were similar to that shown in Figure 1A.…”

Section: Ph and Column Selectionmentioning

confidence: 99%

“…Later HPLC methods using reversed-phase and UV detection, and column switching or amperometric detection [9][10][11][12][13][14][15] has been developed. In order to enhance selectivity and sensitivity, some methods with fluorescence detection are carried out using post-columns [16][17] or pre-columns [19].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a method to determine amoxicillin in physiological fluids using micellar liquid chromatography

Rambla-Alegre

Carda‐Broch

Romero³

2008

J of Separation Science

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Abbreviations MLC micellar liquid chromatographyFDA food and drug analysis 2 Abstract A simple and robust method was developed for the routine identification and quantification of amoxicillin by micellar liquid chromatography. Amoxicillin, a β-lactamase inhibitor, is one of the most commonly prescribed drugs in the treatment of urine and skin structure infections. In this work, amoxicillin was determined in urine samples without any pretreatment step in a phenyl column using a micellar mobile phase of 0.10 M sodium dodecyl sulfate and 4% butanol at pH 3. A UV detection set at 210 nm was used. Amoxicillin is eluted at 5.1 min with no interference by the protein band or endogenous compounds. Linearities (r > 0.9998), intra-and inter-day precisions were determined (RSD (%) 0.4-2.7% and 0.3-5%, respectively in micellar media, and 0.14-2.6% and 0.13-6%, respectively in urine), and robustness was studied in the validation of the method. LOD and LOQ were 0.06 and 0.4 µg/mL in micellar media and 0.11 and 0.4 µg/mL in urine, respectively. Recoveries in the urine matrix were in the range of 95-110%. The validated method proved to be reliable and sensitive for the determination of amoxicillin in urine samples.

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Simultaneous determination of amoxicillin and clavulanic acid in human plasma by isocratic reversed-phase HPLC using UV detection

Cited by 67 publications

References 16 publications

Validated LC-MS/MS Method for the Concomitant Determination of Amoxicillin and Clavulanic Acid from Human Plasma

Validated LC-MS/MS Method for the Concomitant Determination of Amoxicillin and Clavulanic Acid from Human Plasma

Pharmacokinetic parameters of Amoxicillin in Bangladeshi volunteers: a preliminary evaluation

Development and validation of a method to determine amoxicillin in physiological fluids using micellar liquid chromatography

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