Simultaneous determination of amoxycillin and clavulanic acid in pharmaceutical dosage forms by LC with amperometric detection

Aghazadeh, A; Kazemifard, G.

doi:10.1016/s0731-7085(00)00507-0

Cited by 55 publications

(25 citation statements)

References 18 publications

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“…The experiments were performed with a synthetic mixture of amoxicillin and clavulanic acid. The design of the experiment was a factorial design of 2 4 , as shown in the first columns of Table 2. The choice of the domain of the quantitative factors was evaluated from previous experiments and instrumental limitations.…”

Section: Resultsmentioning

confidence: 99%

“…3, where the authors propose the simultaneous determination of amoxicillin and clavulanic acid by multivariate calibration using fluorometric detection; the disadvantage of these methods is that, it involves analyzing many samples in a calibration step. Other papers discuss HPLC with amperometric detection, 4,5 which allows work with interferents, but extends, the analysis time.…”

Section: Introductionmentioning

confidence: 99%

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Sequential Injection Analysis for the Simultaneous Determination of Clavulanic Acid and Amoxicillin in Pharmaceuticals Using Second-order Calibration

Pasamontes

Callao

2006

ANAL. SCI.

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In this paper, we report on a method for quantifying clavulanic acid and amoxicillin simultaneously in pharmaceuticals using sequential injection analysis (SIA) with a diode-array spectrophotometric detector and multivariate curve resolution with alternating least squares (MCR-ALS). We optimized the experimental parameters so that the analytical sequence could distinguish the concentrations and spectrum profiles of the species of interest with optimum resolution quality. After establishing the optimum conditions, we quantified clavulanic acid and amoxicillin in four pharmaceuticals. In most cases our results were slightly higher than those in the prospectus of the pharmaceutical. The relative standard deviations were below 5% for amoxicillin and below 7% for clavulanic acid. These results are acceptable because, to prevent degradation due to bacteriostatic activity, the concentration of the main reactant is usually higher.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequential Injection Analysis for the Simultaneous Determination of Clavulanic Acid and Amoxicillin in Pharmaceuticals Using Second-order Calibration

Pasamontes

Callao

2006

ANAL. SCI.

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“…Usually, high performance liquid chromatographic (HPLC) methods are used for the simultaneous determination of the two substances from pharmaceutical products (Tai-Li et al, 1997; Aghazedeh, Kazemifard, 2001;Pajchel, Pawlowski, Tyski, 2002) and human plasma (Hoizey et al, 2002;Kyung-Hwan et al, 2004;Foroutan et al, 2007), using different special techniques such β-cyclodextrin as stationary phase (Tai-Li et al, 1997), reversed phase RP -HPLC (Foroutan et al, 2007), HPLC -electrospray ionization (ESI) -mass spectrometry (MS) (Kyung-Hwan et al, 2004), with amperometric detection (Aghazedeh, Kazemifard, 2001) or UV detection (Pajchel, Pawlowski, Tyski, 2002;Hoizey et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of amoxicillin and clavulanic acid in pharmaceutical preparations by capillary zone electrophoresis

Hancu

Neacşu

Papp

et al. 2016

Braz. J. Pharm. Sci.

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Clavulanic acid enhances the antibacterial spectrum of amoxicillin by rendering most β-lactamase producing isolates susceptible to the drug. A fast, simple and efficient capillary electrophoresis method was developed for the simultaneous determination of amoxicillin and clavulanic acid from complex mixtures. Using a 25 mM sodium tetraborate as background electrolyte at a pH of 9.30, + 25 kV applied voltage, 25 °C system temperature, UV determination at 230 nm; we succeeded in simultaneous separation of amoxicillin and clavulanic acid in approximately 2 minutes. The analytical performance of the method was evaluated in terms of reproducibility, precision, accuracy, and linearity. The optimized analytical method was applied for the determination of the two analytes from combined commercial pharmaceutical preparations. This CE method is fast, inexpensive, efficient, and environmentally friendly when compared with the more frequently used high performance liquid chromatography methods described in the literature. O ácido clavulânico acentua o espectro antibacteriano de amoxicilina, tornando a maioria dos isolados produtores de β-lactamase sensíveis ao fármaco. Desenvolveu-se um método rápido, simples e eficiente de electroforese capilar (EC) para a determinação simultânea de amoxicilina e de ácido clavulânico a partir de misturas complexas. Usando tetraborato de sódio 25 mM como electrólito em pH de 9,30, voltagem aplicada de + 25 kV, em sistema a 25 ° C e determinação por UV a 230 nm, a foi bem-sucedida a separação simultânea de amoxicilina e ácido clavulânico em, aproximadamente, 2 minutos. O desempenho analítico do método foi avaliado em termos de reprodutibilidade, precisão, exatidão e linearidade. O método analítico otimizado foi aplicado para a determinação dos dois analitos em associação, a partir de preparações farmacêuticas comerciais. Este método de EC é rápido, barato, eficiente e ecologicamente correto, quando comparado aos métodos de cromatografia líquida de alta eficiência mais frequentemente descritos na literatura.Unitermos: Amoxicilina/determinação. Acido clavulânico/determinação. Eletroforese capilar/análise quantitativa. Antibacterianos/análise quantitativa.

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“…Later HPLC methods using reversed-phase and UV detection, and column switching or amperometric detection [9][10][11][12][13][14][15] has been developed. In order to enhance selectivity and sensitivity, some methods with fluorescence detection are carried out using post-columns [16][17] or pre-columns [19].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a method to determine amoxicillin in physiological fluids using micellar liquid chromatography

Rambla-Alegre

Carda‐Broch

Romero³

2008

J of Separation Science

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Abbreviations MLC micellar liquid chromatographyFDA food and drug analysis 2 Abstract A simple and robust method was developed for the routine identification and quantification of amoxicillin by micellar liquid chromatography. Amoxicillin, a β-lactamase inhibitor, is one of the most commonly prescribed drugs in the treatment of urine and skin structure infections. In this work, amoxicillin was determined in urine samples without any pretreatment step in a phenyl column using a micellar mobile phase of 0.10 M sodium dodecyl sulfate and 4% butanol at pH 3. A UV detection set at 210 nm was used. Amoxicillin is eluted at 5.1 min with no interference by the protein band or endogenous compounds. Linearities (r > 0.9998), intra-and inter-day precisions were determined (RSD (%) 0.4-2.7% and 0.3-5%, respectively in micellar media, and 0.14-2.6% and 0.13-6%, respectively in urine), and robustness was studied in the validation of the method. LOD and LOQ were 0.06 and 0.4 µg/mL in micellar media and 0.11 and 0.4 µg/mL in urine, respectively. Recoveries in the urine matrix were in the range of 95-110%. The validated method proved to be reliable and sensitive for the determination of amoxicillin in urine samples.

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Simultaneous determination of amoxycillin and clavulanic acid in pharmaceutical dosage forms by LC with amperometric detection

Cited by 55 publications

References 18 publications

Sequential Injection Analysis for the Simultaneous Determination of Clavulanic Acid and Amoxicillin in Pharmaceuticals Using Second-order Calibration

Sequential Injection Analysis for the Simultaneous Determination of Clavulanic Acid and Amoxicillin in Pharmaceuticals Using Second-order Calibration

Simultaneous determination of amoxicillin and clavulanic acid in pharmaceutical preparations by capillary zone electrophoresis

Development and validation of a method to determine amoxicillin in physiological fluids using micellar liquid chromatography

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