Simultaneous determination of aspartame, acesulfame-K, saccharin, citric acid and sodium benzoate in various food products using HPLC–CAD–UV/DAD

Determining High-Intensity Sweeteners in White Spirits Using an Ultrahigh Performance Liquid Chromatograph with a Photo-Diode Array Detector and Charged Aerosol Detector

“…The LOD for the PDA data were 0.16 µg/g for ACS-K, 0.18 µg/g for DUL, 0.21 µg/g for NHDC, and 0.22 µg/g for SAC. These LOD were much lower than those found in previous studies [9,[19][20][21][22]31] for methods involving HPLC evaporative light-scattering detection and HPLC-MS (13.0 µg/g for ACS-K, 2.0 µg/g for ALI, 10.0 µg/g for ASP, 1.0 µg/g for CYC, 6.0 µg/g for DUL, 5.0 µg/g for NEO, 2.0 µg/g for NHDC, 2.0 µg/g for SAC, and 1.0 µg/g for SCL). This clearly indicates that the method described here was very effective for analyzing sweeteners in white spirits.…”

“…So, LC-MS/MS analyses of sweeteners are more expensive (analysis cost and time) than the ultrahigh performance liquid chromatography (UHPLC) with photo-diode array (PDA) detection and the charged aerosol detection (CAD) method proposed here. Grembecka et al [31] present a combination of HPLC-CAD-UV/DAD detectors to determine three sweeteners (ACS-K, ASP, and SAC) and two preservatives (citric acid and sodium benzoate) in soft drinks, which was a water soluble matrix. Fermented wine contains sweeteners like sulfonamides, dipeptides, and sucrose derivatives, as well as a complex matrix that is water soluble with chemical families, namely esters, alcohols, terpenic compounds, amino acid, and sulphur compounds, etc.…”

Recent Analytical Methods for the Analysis of Sweeteners in Food: A Regulatory Perspective

“…Th is procedure should produce complete dissolution of the candy or tableTh top samples, resulting in transparent solutions with no visual insoluble material remaining aft er shaking. Drink samples are simply diluted with ft H 2 O or mobile phase and filtered with sonication of carbonated beverages fi to remove dissolved gases [25]. Replicate analysis should be performed on all samples and if products are packaged in individual servings (candy, tabletop sweeteners), separate packages should be analyzed.…”

Water-compatible surface imprinting of ‘Saccharin sodium’ on silica surface for selective recognition and detection in aqueous solution

“…There have been reports that the excessive use of saccharin sodium will cause cancer. Various methods including high performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE), and thin layer chromatography have been proposed for the determination of saccharin sodium [20][21][22][23][24][25][26][27][28][29][30]. Instead of these methods, developing a cost-effective saccharin sodium imprinted polymer under water-compatible medium will prove to be a more realistic option for separation and purification of saccharin sodium as well as for its specific and selective measurement in its formulated products.…”