Simultaneous determination of baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re of Banxia xiexin decoction in rat plasma by LC–MS/MS and its application to a pharmacokinetic study

Abstract

A rapid and high-sensitivity liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for simultaneous determination of nine active constituents (baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re) in rat plasma after oral administration of Banxia xiexin decoction (BXD). Biological samples were processed wtih acetone-ethyl acetate (4:1, v/v). The mobile phase consisted of methanol and water (containing 0.1% formic acid) with gradient elution at a flow rate of 0.3 mL/min. Detection was performed on a triple quadrupole mass spectrometer using positive ion and negative ESI in the multiple reaction monitoring mode. The calibration curves for all analytes had good linearity (r > 0.9933). The mean recovery of all the nine active ingredients was >75.2%, and the intra- and inter-day precisions were within 12.0%; the accuracy was between 87.4 and 110.4%. This method was successfully applied to a pharmacokinetic study after administration of BXD. The results of the pharmacokinetic study might be helpful for BXD reasonable clinical application and further studies on mechanism.

A review on analytical methods for natural berberine alkaloids

“…The MS was operated in the positive mode and capillary voltage was 4500 V. All analytes were quantified in multiple reaction monitoring (MRM) mode with transitions of m/z 320.2 to 292.2 for coptisine, m/z 352.3 to 336.3 for palmatine, and m/z 366.3 to 350.2 for dehydrocorydaline . In addition to the specific chemical compounds in the traditional Chinese herbal drugs, pharmacokinetic evaluations of the decoctions and other formulations were also carried out by LC‐MS/MS coupled with TQ‐MS for substances such as Zuojinwan , Erhuang decoction , Jinqi Jiangtang tablets , Gegenqinlian decoction , Shuanghua Baihe tablets , Zi‐Shen pills , and Banxia xiexin decoction . Mass spectrometric detection was performed on different types of TQ‐MS, including the Thermo Finnigan TSQ Quantum TQ‐MS , API 3200 TQ‐MS , API 4000 TQ‐MS , Agilent 6410 TQ‐MS , and Varian 320 TQD MS .…”

Coptisine fromCoptis chinensisexerts diverse beneficial properties: A concise review

“…During the past decades, many pharmacokinetic studies have been focused on the intracorporal process of formulas like SHXXT and BanXia Xiexin decoctions, the main constituents of which were all verified to include coptisine (Chemical structure showed in Figure ). In rat model administrated with SHXXT, coptisine was not metabolized in blood and could reach hepatic and liver cells as prototype form .…”

Ultra‐high‐performance liquid chromatography with tandem mass spectrometry method for determination of four compounds in rat plasma after oral administration of Xanthii fructus and stir‐fried Xanthii fructus extracts

“…The pharmacokinetics of Chinese materia medica (CMM) is defined as the regularity of dynamic change of the absorption, distribution, metabolism and excretion of Chinese herbal active ingredients, components, monomers and compounds as well as their time–dose–time relationship in vivo , and it is also a subject that uses mathematical functions for quantitative description (Hao, Zheng, & Wang, ). Pharmacokinetics study of CMM has become an indispensable link in current research field of traditional Chinese medicine (TCM) (Pei et al, ; Tao et al, ; Wang et al, ; Wang et al, ; Zeng et al, ).…”