Simultaneous Determination of Capilliposide B and Capilliposide C in Rat Plasma by LC–MS/MS and its Application to A PK Study

Abstract

The method was successfully applied to determine the concentrations of capilliposide B and capilliposide C in incurred rat plasma samples, after administration of Lysimachia capillipes Hemsl extract for a rat PK study.

Development and validation of an LC‐MS/MS method for simultaneous quantification of levodopa and MD01 in rat plasma and its application to a pharmacokinetic study of mucuna pruriens extract

“…The method was fully validated in compliance with the guidelines on Bioanalytical Method Validation from the European Medicines Agency (). Linearity, precision, accuracy, matrix effect, recovery, selectivity, sensitivity, carryover, dilution integrity, stability, hemolysis and sample collection stability were evaluated during method validation according to our previously published article (Cheng et al ., ).…”

Development and validation of a sensitive LC–MS/MS assay for the quantification of nizatidine in human plasma and urine and its application to pharmacokinetic study

“…The selection of appropriate elution conditions is pivotal to achieving fast separation and reliable quantitative determination of nizatidine and IS in biological matrices.Of the frequently used organic modifiers, methanol and acetonitrile, we chose a methanol-water mobile phase since the easily dissociated proton of the hydroxyl group of methanol confers superior proton donation character [13]. As summarized in Table 1, an increased proportion of water in the mobile phase resulted in slower elution of analytes.…”

A Rapid LC–MS/MS Quantification of Peramivir Using A Simple and Inexpensive Sample Precipitation: Application to Pk

“…Slight depression of ionization was observed in the response of both analyte and its IS. Matrix effects were also evaluated using IS-normalized MF as described in other reports [25], by dividing the MF of the analyte by the MF of the IS. The IS-normalized MF, determined using six different batches of blank human plasma, ranged from 1.01 to 1.02, indicating compensatory matrix effects for the IS.…”