Simultaneous determination of Cr(III)–EDTA and Cr(VI) by ion interaction chromatography using a C18 column

Threeprom, Jirasak; Purachaka, Sumalee; Potipan, Lamfa

doi:10.1016/j.chroma.2004.09.053

Cited by 41 publications

(15 citation statements)

References 25 publications

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“…Sin embargo, en altas concentraciones es tóxico (Warnick & Bell 1969, Schneider & Jefferson 1997, Natale et al 2000. En cambio, al Cr (VI) no se le conocen acciones benéficas sobre los organismos y se define como tóxico, atribuyéndosele propiedades mutagénicas y cancerígenas (Threeprom et al 2005, Ngah et al 2006, Rai & Mehrotra 2008.…”

Section: Discussionunclassified

“…En cambio, el Cr (VI) sobre pH 7 (agua de mar), podría estar presente como iones cromato, dicromato, cromato ácido y/o ácido crómico (Sperling & Welz 1992), formas aniónicas con muy baja probabilidad de reaccionar con EDTA en solución (Skoog et al 2005, Threeprom et al 2005. Sin embargo, en presencia de EDTA el crecimiento en longitud total de las larvas P. laevigatus fue mayor, en comparación a las larvas sometidas a concentraciones sólo de Cr (VI).…”

Section: Discussionunclassified

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Efecto del EDTA sobre la toxicidad del cromo (VI) en el desarrollo larval de Petrolisthes laevigatus (Guérin, 1835)

Lermanda

Urrutia

Novoa

et al. 2009

Rev. biol. mar. oceanogr.

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Abstract.-The larval development and growth ofPetrolisthes laevigatus were analyzed in five concentrations of Cr VI, 0 (control), 0.25, 0.5, 1, and 5 mg L -1 chrome (VI) in filtered sea water and the same chrome (VI) concentrations, plus 0.7 mg L -1 EDTA. Analyses were done during 21-day period. Measurement of total length, individual wet average larvae weight and the presence or absence of deformations in P. laevigatus larvae were determined. The larvae of the control group (0 mg L -1 chrome (VI)) did not exhibit deformations and developed normally. The total length and biomass of the larvae exposed to Cr VI were significantly lower than those of the larvae subjected to Cr VI plus EDTA concentrations and the control larvae. It is hereby confirmed that the presence of EDTA in the medium mitigates the negative effects of Cr VI via a yet unknown mechanism.Key words: Crab, zoea larva, toxicity, Cr (VI) + EDTA Resumen.-Se analizó el desarrollo larval y el crecimiento de Petrolisthes laevigatus en cinco concentraciones de Cr (VI): 0 (control), 0,25; 0,5; 1 y 5 mg L -1 de cromo (VI) en agua de mar filtrada y las mismas cinco concentraciones de cromo (VI) más la adición de 0,7 mg L -1 de EDTA. El estudio se condujo en 21 días. Se determinó longitud total, peso promedio y presencia o ausencia de malformaciones en larvas de P. laevigatus. Las larvas de las unidades control (0 mg L -1 de cromo (VI)) no presentaron malformaciones y se desarrollaron en forma normal. La longitud total y peso promedio de las larvas expuestas a Cr (VI) fueron significativamente menores con respecto a los de las larvas sometidas a las concentraciones de Cr (VI) más EDTA y a las unidades control. Se confirma que la presencia de EDTA en el medio atenúa los efectos negativos del Cr (VI) por un mecanismo aún no dilucidado.Palabras clave: Cangrejo, larva zoea, toxicidad, Cr (VI) + EDTA IntroducciónLos metales vertidos en fuentes de agua han tenido efectos negativos, en distintos grados, en el crecimiento, supervivencia y desarrollo de las especies que allí habitan. Principalmente son las primeras etapas del ciclo de vida de los organismos las que sufren los efectos negativos, en el tamaño de la primera reproducción, número de episodios reproductivos, ontogenia en las fases posteriores y longevidad de los organismos (Hadfield & Strathmann 1996, Natale et al. 2000, Boada et al. 2007. Es así que resulta preocupante el aumento de residuos con metales eliminados en la zona costera y el efecto que puede causar en las especies y el medioambiente.El cromo es un metal que ingresa como integrante de residuos líquidos al medio acuático, debido a su utilización en actividades tan diversas como: la industria textil, cueros, industrias de papel y cartón, petroquímica, abonos, refinerías, metalúrgica, vidrios, cementos, asbestos, pinturas, cerámicas, fungicidas, catálisis y galvanizados (Bodar et al. 1990, Rengaraj et al. 2001).Por ejemplo, un área que representa un segmento importante de la economía chilena, como Bahía San Vicente, recibiría a través de los eflue...

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Section: Discussionunclassified

Efecto del EDTA sobre la toxicidad del cromo (VI) en el desarrollo larval de Petrolisthes laevigatus (Guérin, 1835)

Lermanda

Urrutia

Novoa

et al. 2009

Rev. biol. mar. oceanogr.

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“…The widely used techniques for the following determination of separate chromium species include above all graphite furnace atomic absorption spectrometry (GFAAS) [9], flame atomic absorption spectrometry (FAAS) [10] and inductively coupled plasma mass spectrometry (ICP-MS) [11]. In the literature data combined methods for simultaneous determination of Cr(VI) and Cr(III) are also described, e.g., ion interaction chromatography with UV detection [12,13], ion chromatography with ICP-MS [14]. However, detection limits of Cr(VI) determination in these methods are often unsatisfactory and, moreover, these combined procedures do not meet the requirements of routine analysis, principally because of their complicated design and expensive cost.…”

Section: Introductionmentioning

confidence: 99%

Speciation Analysis of Chromium by Adsorptive Stripping Voltammetry in Tap and River Water Samples

Grabarczyk

2008

Electroanalysis

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An adsorptive stripping voltammetric method for speciation analysis of chromium in natural water samples has been developed. Ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) were used as complexing agents for Cr(III) present in the sample and formed as products of Cr(VI) reduction, respectively. Under optimum experimental conditions linear relations in the range from 1 Â 10 À6 to 3 Â 10 À5 mol L À1 without accumulation and from 1 Â 10 À9 to 1 Â 10 À7 at 30 s accumulation time were obtained for Cr(III) and Cr(VI), respectively. For samples in which Cr(III) concentration is higher than 1 Â 10 À6 mol L À1 the Cr(III) and Cr(VI) were determined simultaneously in one voltammetric cell. For samples in which Cr(III) concentration is below 1 Â 10 À6 mol L À1 only Cr(VI) was selectively determined in the presence of Cr(III), which did not influence the Cr(VI) signal. The determination of Cr(III) and Cr(VI) was successful with the application of the proposed procedure in the presence of common foreign ions. The presented method was applied for the speciation of chromium in spiked tap and river water samples with satisfactory results.

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“…and thus improves the sensitivity of chromium analysis [9]. The chromatographic methods for chromium speciation analysis may involve either ion chromatography (IC) [10,11] or ion-pair reversed-phase chromatography (RPIPC) [12,13]. RPIP-HPLC is widely used as a simple, flexible and sensitive method for separating chromium compounds.…”

Section: Introductionmentioning

confidence: 99%

Speciation analysis of chromium in drinking water samples by ion-pair reversed-phase HPLC–ICP-MS: validation of the analytical method and evaluation of the uncertainty budget

et al. 2013

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The approach presented in this article refers to the modification of a method for the detection and quantitative determination of chromium species in water by high-performance liquid chromatography inductively coupled plasma mass spectrometry. The main aim of this work was to establish a detailed validation of the analytical procedure and an estimation of the budget of measurement uncertainty which was helpful in recognizing the critical points of the presented method. As a result of the method validation experiment, the obtained limit of quantification, repeatability and intermediate precision were satisfied for the quantification Cr(III) and Cr(VI) in water matrices. The trueness of the method was verified via an estimation of the recovery of the spiked real samples. The recovery rate of both determined analytes was found to be between 93 and 115 %. Considering that the validation of the method and the evaluation of measurement uncertainty are crucial for quantitative analysis, the above-mentioned assessment of the uncertainty budget was performed in two different ways: a modelling approach and a single-laboratory validation approach. The measurement uncertainties of the results were found to be 4.4 and 7.8 % for Cr(III), 4.2 and 7.9 % for Cr(VI) using the classical concept and method validation data, respectively. This paper is the first publication to presenting all the steps needed to evaluate the measurement uncertainty for the speciation analysis of chromium species. In summary, the obtained results demonstrate that the method can be applied effectively for its intended use.

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Simultaneous determination of Cr(III)–EDTA and Cr(VI) by ion interaction chromatography using a C18 column

Cited by 41 publications

References 25 publications

Efecto del EDTA sobre la toxicidad del cromo (VI) en el desarrollo larval de Petrolisthes laevigatus (Guérin, 1835)

Efecto del EDTA sobre la toxicidad del cromo (VI) en el desarrollo larval de Petrolisthes laevigatus (Guérin, 1835)

Speciation Analysis of Chromium by Adsorptive Stripping Voltammetry in Tap and River Water Samples

Speciation analysis of chromium in drinking water samples by ion-pair reversed-phase HPLC–ICP-MS: validation of the analytical method and evaluation of the uncertainty budget

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