Simultaneous Determination of Epinephrine and Uric Acid at Poly (Guanine) Modified Glassy Carbon Electrode

Abstract

An electrochemical sensor based on poly (guanine) modified glassy carbon electrode (PGA/GCE) was fabricated by electropolymerization of guanine on a bare GCE surface. Scanning electron microscope (SEM) was employed to characterized the electrode surface. This modified electrode exhibited a good electrocatalytic property towards the oxidation of epinephrine (EP) and uric acid (UC) in 0.1 M phosphate buffer solution (PBS) (pH 4.0) due to the enhanced peak currents and well-defined peak separations. Under optimum reaction conditions, the oxidation peak currents of EP and UA were proportional to their concentrations in the ranges of 1.0 × 10 −5 to 1.0 × 10 −3 M for both two compounds, and the detection limits were 1.8 × 10 −6 and 5.0 × 10 −7 M, respectively. Finally, this method was efficiently used for the determination of EP in EP injections.

Simultaneous Electrochemical Determination of Articaine HCl and Epinephrine

“…The mean percentage recoveries were of ) as a function of scan rate. [22], 1.143-142.90 μg mL -1 [23], and 1.8-35.3 μg mL -1 [24], and electrochemical methods for epinephrine: 3.0-175.0 μmol L −1 [26], and 1.0 × 10 −5 to 1.0 × 10 −3 mol L −1 [29]. The regression parameters were computed and presented in Table 1.…”

“…This allows the local anesthetic to persist at the injection site before being absorbed into the systemic circulation [4]. Literature survey reveals that several different analytical techniques have been developed for the determination of articaine HCl and epinephrine including high performance liquid chromatographic methods [5][6][7], thin layer chromatographic methods [8], gas chromatographic methods [9] and spectrophotometric methods [10] for articaine HCl and high performance liquid chromatographic methods [11][12][13][14][15], thin layer chromatographic methods [16,17], gas chromatographic methods [18,19], spectrophotometric methods [20][21][22][23][24] and electrochemical methods [25][26][27][28][29][30] for epinephrine. Carbon based electrodes are currently in widespread use in electroanalytical chemistry, because of their broad potential window, low cost, rich surface chemistry, low background current and chemical inertness.…”

Voltammetric and amperometric sensors for determination of epinephrine: A short review (2013-2017)

“…In their study, Li and Wang [54] have illustrated that K + , Na + , Ca 2+ , Mg 2+ , sucrose and glucose do not interfere significantly, while L-glutamic acid, Cu 2+ and Fe 2+ ions showed a certain effect on the examinations of EP and UA.…”

“…Li and Wang [54] have investigated an electrochemical sensor based on the poly(guanine) (PGA) modified GCE that was fabricated by electropolymerization of guanine on the bare GCE surface. This modified electrode exhibited good electrocatalytic property towards the oxidation of EP and UA in 0.1 mol L -1 PBS (pH 4.0), seen as enhanced peak currents and well defined peak separations.…”