Simultaneous determination of febantel, fenbendazole, oxfendazole and oxfendazole sulfone in livestock by matrix solid phase dispersion extraction technique and HPLC

Su, Shu-Chu; Chou, H.-H.; Chang, P.-C.; Liu, Chiung‐Hui; Chou, Shin-Shou

doi:10.38212/2224-6614.2630

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…Generally, aqueous extraction method is used in which high pH causes partitioning into an immiscible organic solvent [15,16]. Some of the samples are pre-treated using either matrix solidphase dispersion method (MSPD), [23] or supercritical fluid extraction method [24] with multiple stages of liquid-liquid extraction and/or solid-phase extraction method [25]. Besides these tedious and time-consuming sample treatments, chiral analysis by an indirect method in the enantiomer selective assays of (±)-ABZSO was also performed, in which the fractions of different enantiomers (±) were collected.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Dual Column HPLC Method for Determination of Albendazole and Its Metabolites in Rat Plasma

Dey¹,

Mandal²,

Bhowmick³

et al. 2021

Egypt. J. Chem.

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To develop a quick, simple and reproducible dual column high performance liquid chromatography (HPLC) method to determine the albendazole and its metabolites in rat plasma. Albendazole (ABZ), albendazole sulfoxide (ABZSO) and albendazole sulfone (ABZSO2) were analyzed in rat plasma by high performance liquid chromatography using UV-detector. Preparation of plasma samples was carried out by protein precipitation using 8.25% perchloric acid. This method involves two different mobile phases with two different columns and different wavelengths. Estimation of Albendazole was done using Enable C18 column (250 mm × 4.6 mm, 5μm: SpinCo Biotech Pvt. Ltd.), mobile phase acetonitrile: water in the ratio 60: 40, wavelength 225nm and Praziquantel as an internal standard (IS). The retention time for Albendazole and Praziquantel was 3.7 and 6.4 minutes respectively. But the estimation of Albendazole Sulfoxide, Albendazole Sulfone were done by using Phenomenex C18 Luna column (250 mm × 4.6 mm, 5μm: USA), mobile phase acetonitrile: methanol: phosphate buffer (20mM) in the ratio 20: 25: 55. The pH was adjusted to 6.9 using 0.1N NaOH solution, wavelength 290nm and oxfendazole an internal standard (IS). The retention time for Albendazole Sulfoxide, Albendazole Sulfone, and Oxfendazole was 5.5, 7.0 and 8.2 minutes respectively. Both the methods were validated over the range from 0.005-5µg/mL for Albendazole, 0.05-80µg/mL for Albendazole Sulfoxide and Albendazole Sulfone. Both the method showed % RSD and % DEV lower than 15% for all the analytes. The limit of quantitation was 0.005µg/mL for Albendazole whereas 0.05µg/mL for Albendazole Sulfoxide and Albendazole Sulfone. Metabolites of albendazole were analyzed in rat plasma samples using a single dose of Albendazole 50mg/kg was determined application of this method was also used to found the pharmacokinetic studies.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of a Dual Column HPLC Method for Determination of Albendazole and Its Metabolites in Rat Plasma

Dey¹,

Mandal²,

Bhowmick³

et al. 2021

Egypt. J. Chem.

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“…Literature shows few HPLC methods for quantification of febantel from veterinary preparations [8,9], feeds [10]. There are liquid chromatographic methods for determination of febantel in biological matrices [11][12][13][14][15] and fate of anthelmintics in environment [16,17]. Various assay methods are also available based on determination by LC and GC-MS [18], HPLC-MS/MS [19][20][21], UV spectrophotometry [22,23], IR, thermogravimetry, and scanning electron microscopy [24,25].…”

Section: Introductionmentioning

confidence: 99%

Characterization and toxicity evaluation of degradation products of febantel

Mahajan¹,

Marathe²,

Jarande³

et al. 2020

Futur J Pharm Sci

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Background The aim of the present work was to determine potential toxicity of degradation products of febantel generated under different stress conditions mentioned in guideline Q1A (R2) laid down by International Council for Harmonization (ICH). The stability behavior of febantel was studied by subjecting it to hydrolytic, oxidative, photolytic and thermal forced degradation conditions. Results Five degradation products (DPs) were observed which were resolved using high-performance liquid chromatography (HPLC) and characterized by LC-MS/MS using positive mode of electrospray ionization. The chromatographic separation was carried out on Hypersil® BDS C18 (150 × 4.6 mm, 5 μm) column. Optimum resolution was obtained using ammonium formate buffer (10 mM, pH 3.5) and acetonitrile programmed in gradient elution mode at 281.0 nm using photodiode array detector. Conclusion The drug was found susceptible to degradation under all the stress conditions except thermal and oxidative stress. Five major unknown degradation products DP–I, DP–II, DP–III, DP–IV, and DP–V generated under photolytic, alkali, and acidic stress condition were identified and characterized by LC-MS/MS. The drug and identified degradation products were screened for prediction of in-silico toxicity using software viz. Swiss ADME, OSIRIS Property Explorer and Pro Tox II which indicated overall no toxicological concerns. Graphical abstract

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“…2 Different analytical techniques were applied for quantitative estimation of oxfendazole including potentiometric titration, 3,4 radio immunoassay, 5 spectrophotometric methods 6 and HPLC methods even alone or in presence of other compounds. [7][8][9][10][11][12][13][14][15][16][17] Oxfendazole is an amide group containing compound that made it highly sensitive to hydrolytic degradation in basic conditions with the production of the degradation product 2-amino-5-Phenylsulfinyl benzimidazole. There is no stability indicating analytical methods were reported for determination of oxfendazole in presence of its degradation product.…”

Section: Introductionmentioning

confidence: 99%

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

Salama¹,

Attia²,

Mohamad³

et al. 2018

JAPLR

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Accurate, selective, and sensitive reversed phase high performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-densitometry methods have been developed and validated for determination of oxfendazole in the presence of its alkaliinduced degradation product. The developed RP-HPLC method was based on the separation of the two components using 0.05M KH 2 PO 4 (adjusted to pH=5), methanol and actonitrile (50:30:20, by volume) as a mobile phase in isocratic elution mode on BDS Hypersil C 18 column at a flow rate of 1mL min −1 and ultraviolet (UV) detection at 225nm. The components were well resolved from each other with significantly different R t values of 7.3 and 4.05min for oxfendazole and its alkali-induced degradation product, respectively. The method was applied in the range of (3-15µg mL-1). On the other hand, TLC-densitometric method depends on the separation and quantitation of oxfendazole and its alkali-induced degradation product on TLC silica gel 60 F254 plates, using chloroformmethanol and glacial acetic acid (90:8:2, by volume) as the developing system followed by densitometric measurement at 292nm. The studied components were well resolved from each other with significantly different R f values of 0.75 and 0.2 for oxfendazole and its degradation product, respectively. The method was applied in the range of (0.5-6µg / spot). The developed methods were validated according to the International Conference on Harmonization (ICH) guidelines demonstrating good accuracy and precision. The results were statistically compared with those obtained by the reported method, and no significant difference was found.

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Simultaneous determination of febantel, fenbendazole, oxfendazole and oxfendazole sulfone in livestock by matrix solid phase dispersion extraction technique and HPLC

Cited by 5 publications

References 13 publications

Development and Validation of a Dual Column HPLC Method for Determination of Albendazole and Its Metabolites in Rat Plasma

Development and Validation of a Dual Column HPLC Method for Determination of Albendazole and Its Metabolites in Rat Plasma

Characterization and toxicity evaluation of degradation products of febantel

RP- HPLC and TLC- densitometric methods for determination of oxfendazole in the presence of its alkali -induced degradation product

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