Simultaneous determination of five anthraquinones in medicinal plants and pharmaceutical preparations by HPLC with fluorescence detection

He, Dongxiu; Chen, Bo; Tian, Qingqing; Yao, Shanshan

doi:10.1016/j.jpba.2009.02.014

Cited by 78 publications

(46 citation statements)

References 32 publications

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“…Reagent grade solvents were used for extraction; HPLC grade solvents were employed for chromatographic analysis. HPLC analysis was performed by JASCO system, using 250×4.6 mm, RP-18 (5-μm particle size) Purospher star column (Merck), with a flow rate of 1.00 ml/min, and monitoring was done using a Jasco UV-1575 detector and a JASCO FP-2020 Plus Intelligent fluorescence detector in series, with an isocratic elution program of methanol : water containing 0. emission wavelengths of 440 and 540 nm (for fluorescence detection) were employed (He et al 2009). …”

Section: General Experimental Proceduresmentioning

confidence: 99%

Assessment of conventional and novel extraction techniques on extraction efficiency of five anthraquinones from Rheum emodi

2015

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Rheum emodi is principally known to consist 1,8-dihydroxyanthraquinones (DHAQs) that find immense use in the chemical, pharmaceutical, cosmetic industries and in herbal medication and food sector. The aim of this study was to compare non-conventional and classical methods for extraction of anthraquinones from R. emodi. Optimisation of the extraction parameters for various methods was done and their extraction efficiency was evaluated. In preliminary screening experiments, choice of solvent and solid : solvent ratio was optimised. Comparison of extraction efficiency for classical methods like maceration, heat-reflux, soxhletion and nonconventional methods like ultra-sonication and sublimation was done for five DHAQs -aloe emodin, rhein, emodin, chrysophanol and physcion using HPLC-UVand fluorescence detection in native and acid hydrolysed samples. It was observed that ethanol was the best solvent for extraction of anthraquinones with a solid : solvent ratio of 1:20. A prior acid hydrolysis led to significant increase in anthraquinone extraction. Among the extraction methods heat reflux for 45 min was the most prominent extraction method with highest recovery of the DHAQs. In ultrasonic assisted extraction, an increase in the anthraquinone extraction was seen till 45 min after which the concentration declined. A novel, solvent-free, green and selective method of extraction by sublimation was found to be effective for extraction of anthraquinones.

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Section: General Experimental Proceduresmentioning

confidence: 99%

Assessment of conventional and novel extraction techniques on extraction efficiency of five anthraquinones from Rheum emodi

2015

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“…Recently, a number of pharmacological tests revealed that the anthraquinone derivatives present various biological activities including antifungal (S K. Agarwal, 2000, as cited in He, 2009) He, 2009). In general, fluorescence detection is sensitive and selective.…”

Section: Pharmaceutical Analysismentioning

confidence: 99%

“…The five anthraquinones are known to possess natural fluorescence, but it is difficult to analyse and determine their contents by conventional fluorimetry due to their similar molecule structures. Therefore, recently a simple, rapid and sensitive reversed-phase highperformance liquid chromatography (RP-HPLC) method, using fluorescence detection, to simultaneously quantify aloe-emodin, emodin, rhein, chrysophanol and physcion in medicinal plants and their pharmaceutical preparations was developed by He et al (He et al, 2009). Their method was suitable for use as a tool for routine quality assurance and standardization of the anthraquinone from the raw material and commercially available pharmaceutical preparations containing rhubarb.…”

Section: Pharmaceutical Analysismentioning

confidence: 99%

Current Achievement and Future Potential of Fluorescence Spectroscopy

Al-Rawashdeh¹

2012

Macro to Nano Spectroscopy

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“…HPLC coupled with ultraviolet-visible, photodiode array, fluorescence, or mass spectrometry (MS) detectors are most frequently used for anthraquinones (He et al 2009;Shi et al 2007;Krizsán et al 1996;Yi et al 2007;Ding, Ma, and Liu 2003;Song et al 2010).…”

Section: Introductionmentioning

confidence: 99%

A Novel Rhein-Functionalized Resin with Application for the Preconcentration of Anthraquinones

et al. 2014

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Anthraquinones are active components of herbal medicine, and consequently it is necessary to develop a selective, convenient, and green preconcentration method for these compounds. A rhein-functionalized resin was synthesized by a condensation reaction followed by characterization by infrared spectroscopy, transmission electron microscopy, solid carbon-13 nuclear magnetic resonance, and elemental analysis. The rhein-functionalized resin was employed for solid-phase extraction with HPLC-MS for the determination of anthraquinones in the extracts of Polygonum cuspidatum. Emodin, chrysophanol, and physcion were selectively concentrated by a simple absorption and desorption procedure. The results indicated that this new material has application for separating and enriching anthraquinones from herbal medicine, which is significant for pharmaceutical analysis.

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Simultaneous determination of five anthraquinones in medicinal plants and pharmaceutical preparations by HPLC with fluorescence detection

Cited by 78 publications

References 32 publications

Assessment of conventional and novel extraction techniques on extraction efficiency of five anthraquinones from Rheum emodi

Assessment of conventional and novel extraction techniques on extraction efficiency of five anthraquinones from Rheum emodi

Current Achievement and Future Potential of Fluorescence Spectroscopy

A Novel Rhein-Functionalized Resin with Application for the Preconcentration of Anthraquinones

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