Simultaneous determination of fluconazole and ofloxacin in rabbit tear fluid by LC-MS/MS: Application to ocular pharmacokinetic studies

Simultaneous pharmacokinetic assessment of phytopharmaceuticals in fenugreek extract using LC–MS/MS in Sprague–Dawley rats

“…Blank plasma sample was used as the biomatrix for evaluation of the analytes under various storage conditions. The method was validated for selectivity, sensitivity, linearity, precision, accuracy, matrix effect, recovery, dilution integrity, carryover effect, and stability (Imre et al, 2008; Sanap, Mishra, et al, 2022).…”

Codetermination of antimicrobial agents in rabbit tear fluid using LC–MS/MS assay: Insights into ocular pharmacokinetic study

“…2,[5][6][7] Within the spectrum of fungal keratitis (FK), those attributable to Candida are the most widespread, accounting for approximately one million new corneal infections annually. 8 There is an observed correlation between a severe ocular fungal infection and the concurrent development of a bacterial infection at the same site, contributing to the emergence of PMK. 9 The primary causes of PMK involve prolonged and irrational utilization of chemotherapeutic agents and immunosuppressants.…”

“…Few studies have looked at the causes and symptoms of polymicrobial corneal infections, and many have looked at the effects of bacterial and fungal coexistence in corneal infections 2,5–7 . Within the spectrum of fungal keratitis (FK), those attributable to Candida are the most widespread, accounting for approximately one million new corneal infections annually 8 . There is an observed correlation between a severe ocular fungal infection and the concurrent development of a bacterial infection at the same site, contributing to the emergence of PMK 9 .…”

Simultaneous estimation of voriconazole, moxifloxacin, and pirfenidone in rabbit lacrimal matrix using LC-MS/MS: an application to preclinical ocular pharmacokinetics

“…A few microliters of lacrimal sample (∼3-10 mL) with an ultrasensitive quantitation method were sufficient to monitor the subtherapeutic drug levels and establish a related dosage regimen. 36,37 Lacrimal sample extraction using solid-phase and liquid-liquid extraction is a very expensive and chronophagous task. Hence, herein in this work, we have adopted a simple protein precipitation technique to extract the analytes from the lacrimal matrix and developed a simple and sensitive LC-MS/ MS method for the simultaneous quantication of VCZ, MOXI, and PIR from rabbit lacrimal uid.…”