Simultaneous Determination of Four Index Polar Constitutes by HILIC-ESIMS/ MS for the Quality Control of Guizhi Fuling Wan

Abstract

A rapid and accurate method was developed and validated for the analysis of four index polar constitutes in Chinese medicinal preparation Gui-zhi-fu-ling Wan by microwave-assisted extraction coupled with hydrophilic interaction liquid chromatography-tandem mass spectrometry (MAE-HILIC -MS/MS). The chromatographic separation was performed on a Waters HILIC column (100 mm × 1.7 mm, 2.1 μm) with gradient elution using 0.1% formic acid aqueous solution (A) and acetonitrile (B) at a flow rate of 0.2 mL/min. All the compounds showed good linearity. The method provided good accuracy with perfect recoveries and precision, which has been successfully applied to the simultaneous identification and determination of 4 index compounds in a single run for the quality control of Gui-zhi-fu-ling Wan.

Quality and Reliability in Analytical Chemistry

“…Author Zhang et al had developed and validated a rapid and accurate method for the analysis of four-index polar constitutes practiced in Chinese medicinal preparation 'Gui-zhi-fu-ling Wan' by microwave-assisted extraction coupled with hydrophilic interaction, coupled with liquid chromatography-tandem mass spectrometry (MAE-HILIC -MS/MS). This method has been successfully applied for the identification and determination of 4 index compounds in a single run to maintain the quality of Gui-zhi-fu-ling Wan [2].…”