Simultaneous Determination of Four Marker Compounds in Lobelia chinensis Lour. Extract by HPLC-PDA

Abstract

Lobelia chinensis Lour. (L. chinensis) has traditionally been used as a treatment for snake bites, high fever, jaundice, edema, and diarrhea, and modern studies have reported its anti-inflammatory, antioxidant, and antiviral activities. L. chinensis contains various compounds, such as flavonoids and coumarins, and its flavonoid components have been identified in many studies. In this study, a high-performance liquid chromatograph equipped with a photodiode array (PDA) detector and an Aegispak C18-L reverse-phase column (4.6 mm × 250 mm i.d., 5 μm) was used to simultaneously analyze four marker components in L. chinensis for standardization purposes. HPLC-PDA (detection at 340 nm), performed using a 0.1% formic acid-water/0.1% formic acid-acetonitrile gradient, separated the four marker compounds: luteolin-7-O-β-d-glucuronopyranosyl (1→2)-O-β-d-glucuronopyranoside, clerodendrin, chrysoeriol-7-O-diglucuronide, and diosmin. The developed analytical method showed excellent linearity values (r2 > 0.9991), limits of detection (LODs: 0.376–2.152 μg/mL), limits of quantification (LOQs: 1.147–6.521 μg/mL), intra- and inter-day precisions (RSD < 1.96%), and analyte recoveries (96.83–127.07%; RSD < 1.73%); thus, it was found to be suitable for the simultaneous analysis of these four marker compounds in L. chinensis.

Lobelia chinensis Extract and Its Active Compound, Diosmetin, Improve Atopic Dermatitis by Reinforcing Skin Barrier Function through SPINK5/LEKTI Regulation

“…Thus, three flavonoids (diosmin, linarin, and diosmetin) and two coumarins (tomentin and 6,8-dimethoxycoumarin) were detected ( Figure 3 B,C). In the previous experiment, HPLC-PDA results of L. chinensis water extract revealed that diosmetin was one of the marker compounds [ 31 ]. When we evaluated the effects of these five components by assessing the transcriptional activity of SPINK5 using a dual-luciferase assay, only diosmetin increased its transcriptional activity ( Figure 3 D); this showed that diosmetin was responsible for the increased expression of SPINK5 induced by LCE.…”

HPLC-PDA Method for Quantification of Bioactive Compounds in Crude Extract and Fractions of Aucklandia costus Falc. and Cytotoxicity Studies against Cancer Cells

“…The sensitivity of the method was evaluated in terms of limits of detection (LODs) and limits of quantification (LOQs) [ 49 ]. The equations for their calculation areas follows: LOD = 3 Sn/u and LOQ = 10 Sn/u, where u is the slope of the calibration curve, and Sn is the standard deviation of the y-intercept [ 50 ]. Precision data were calculated for both inter- and intraday analysis.…”

“…Precision data were calculated for both inter- and intraday analysis. The intraday precision test was carried out by analyzing three different concentrations (Low, Medium and High) of each compound injected thrice on the same day, while interday precision was obtained by injecting the same three concentrations in triplicate on three consecutive days [ 50 , 51 ]. Recovery studies of the analytes were performed so as to confirm their percentage recoveries [ 52 ].…”