Simultaneous determination of fourteen components of Gumiganghwal-tang tablet in human plasma by UPLC-ESI-MS/MS and its application to pharmacokinetic study

Jeong, Seung‐Hyun; Jang, Ji-Hun; Lee, Guk-Yeo; Yang, Seung-Jung; Cho, Hea-Young; Lee, Yong-Bok

doi:10.1016/j.jpha.2020.08.003

Cited by 11 publications

(9 citation statements)

References 23 publications

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“…29 According to our current study, the CL/Fz data of atractylenolide III, ferulic acid and geniposide were not more than 500 mL h −1 kg −1 , while the CL/Fz data of hesperidin, naringin and puerarin were more than 90 000 mL h −1 kg −1 . Compared with the literature, 28,30–34 the results indicate that some metabolic enzymes in the depressed rats were activated by acute stress, while some were inhibited, highlighting the value of studying the metabolism of depression in vivo and in vitro . Thus, our future research will focus on the metabolism of XYSJW and compatibility mechanism using depressed rats and rat liver microsomes.…”

Section: Resultsmentioning

confidence: 79%

Identification of absorbed compounds of Xiao Yao San Jia Wei and pharmacokinetic study in depressed rats by force swimming stress

et al. 2022

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Section: Resultsmentioning

confidence: 79%

Identification of absorbed compounds of Xiao Yao San Jia Wei and pharmacokinetic study in depressed rats by force swimming stress

et al. 2022

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“…137.14 mg/kg). The rat’s oral dose was calculated as the ratio of body weight based on the maximum daily dose (9.6 g) in humans; it was established that 137.14 mg/kg of Gumiganghwal‐tang can be administered to rats when 9.6 g is administered based on the approximate average weight of 70 kg (for men) (Jeong et al, 2020). All other experimental procedures and sampling points (including sample matrices) were identical to the PK studies of standards (as mentioned in the “Application to the Pharmacokinetic Studies of Standards” section).…”

Section: Methodsmentioning

confidence: 99%

Simultaneous determination of asarinin, β‐eudesmol, and wogonin in rats using ultraperformance liquid chromatography–tandem mass spectrometry and its application to pharmacokinetic studies following administration of standards and Gumiganghwal‐tang

Jeong

Jang

Cho

et al. 2020

Biomedical Chromatography

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Asarinin, β‐eudesmol, and wogonin have common antiangiogenic activities and have the potential for use in chemotherapy. Besides, they are multivalent substances that are combined in various herbal medicines. The purpose of this study was to develop a method for simultaneous analysis of asarinin, β‐eudesmol, and wogonin, which are representative pharmacological components of Asarum heterotropoides, Atractylodes lancea, and Scutellaria baicalensis, respectively, in rat biosamples using ultraperformance liquid chromatography–tandem mass spectrometry. The three components were separated using 5 mm aqueous ammonium acetate containing 0.1% formic acid and acetonitrile as a mobile phase, equipped with a KINETEX core‐shell C18 column. The analysis was quantitated on a triple‐quadrupole mass‐spectrometer employing electrospray ionization, and operated in the multiple reaction monitoring mode. The chromatograms showed high resolution, sensitivity, and selectivity with no interference with plasma, urine, and feces constituents. The developed analytical method satisfied international guidance criteria and could be successfully applied to the pharmacokinetic (PK) studies evaluating oral bioavailability of asarinin, β‐eudesmol, and wogonin after oral and intravenous administration and their urinary and fecal excretion ratios after oral administration to rats. Furthermore, the analysis was extended to PK studies following oral administration of Gumiganghwal‐tang. This study was the first simultaneous analysis of the aforesaid three constituents in rat plasma, urine, and feces that also determined their PK parameters.

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“…Like other traditional herbal medicines made from an average of more than 7-10 kinds of herbs, SCRT tablets contain a mixture of eight different herbs [7]. Therefore, the herbal medicines administered in multiple doses can exhibit different pharmacokinetic parameters in the body compared to single-dose administrations due to the interaction between the components or the effect of certain components contained in the formulation on the gastrointestinal tract [13,14]. For example, ephedrine, a marker component of Ephedrae Herba, is known to suppress gastric acid secretion and gastrointestinal motility [48,49]; and cinnamic acid, an indicator component of Cinnamoni Ramulus, has been reported to inhibit gastrointestinal motility and spasms in mice [50].…”

Section: Parametersmentioning

confidence: 99%

“…Thus, pharmacokinetic studies are essential to more efficiently evaluate the safety and efficacy of SCRT. In addition, based on the pharmacokinetic results, the dosage of the drug required to reach blood concentrations within the therapeutic range can be determined [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…However, there have been no reports on the pharmacokinetic results of multiple doses of SCRT. Traditional herbal medicines such as SCRT tend to be used more than once because of the relatively lower side effects and the chronic use for disease treatment compared to chemical or biological drugs, and medication compliance is relatively high [14,16]. Moreover, for SCRT tablets, the approved clinical regimen is oral administration three times a day [7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pharmacokinetic Changes According to Single or Multiple Oral Administrations of Socheongryong-Tang to Rats: Presented as a Typical Example of Changes in the Pharmacokinetics Following Multiple Exposures to Herbal Medicines

et al. 2021

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The purpose of this study was to investigate the pharmacokinetic properties of ephedrine, paeoniflorin, and cinnamic acid after single or multiple doses of Socheongryong-tang (SCRT) were administered to rats, and to present an example of the pharmacokinetic changes following multiple doses of an herbal medicine. SCRT is a traditional herbal medicine that has been used clinically for a long time, and its main ingredients include ephedrine, paeoniflorin, and cinnamic acid. However, studies on the pharmacokinetic properties of SCRT are insufficient, and particularly, no pharmacokinetic information has been reported for multiple doses. In this study, SCRT was administered orally to rats once or multiple times, and plasma sampled at different times was quantitatively analyzed for ephedrine, paeoniflorin, and cinnamic acid using ultra-high-performance liquid chromatography-tandem mass spectrometry. There was a difference between the pharmacokinetic parameter values of each component (especially in paeoniflorin and cinnamic acid) obtained after single or multiple doses of SCRT. The actual observed values of each component obtained after multiple doses of SCRT were clearly different from the predicted results of multiple-dose simulations based on the pharmacokinetic profiles obtained after a single dose. The results confirmed that the plasma concentrations and, thus, exposures to paeoniflorin and cinnamic acid were significantly increased when SCRT was administered multiple times, whereas that of ephedrine was not. The results of this study are expected to provide useful pharmacokinetic data for the safety and efficacy evaluation of SCRT in the future and demonstrate the necessity of pharmacokinetic comparison studies according to single or multiple oral administrations of herbal medicines.

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Simultaneous determination of fourteen components of Gumiganghwal-tang tablet in human plasma by UPLC-ESI-MS/MS and its application to pharmacokinetic study

Cited by 11 publications

References 23 publications

Identification of absorbed compounds of Xiao Yao San Jia Wei and pharmacokinetic study in depressed rats by force swimming stress

Identification of absorbed compounds of Xiao Yao San Jia Wei and pharmacokinetic study in depressed rats by force swimming stress

Simultaneous determination of asarinin, β‐eudesmol, and wogonin in rats using ultraperformance liquid chromatography–tandem mass spectrometry and its application to pharmacokinetic studies following administration of standards and Gumiganghwal‐tang

Pharmacokinetic Changes According to Single or Multiple Oral Administrations of Socheongryong-Tang to Rats: Presented as a Typical Example of Changes in the Pharmacokinetics Following Multiple Exposures to Herbal Medicines

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